PMID- 26082777
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150617
LR  - 20220131
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 6
DP  - 2015
TI  - Investigating Evolutionary Conservation of Dendritic Cell Subset Identity and 
      Functions.
PG  - 260
LID - 10.3389/fimmu.2015.00260 [doi]
LID - 260
AB  - Dendritic cells (DCs) were initially defined as mononuclear phagocytes with a 
      dendritic morphology and an exquisite efficiency for naive T-cell activation. DC 
      encompass several subsets initially identified by their expression of specific 
      cell surface molecules and later shown to excel in distinct functions and to 
      develop under the instruction of different transcription factors or cytokines. 
      Very few cell surface molecules are expressed in a specific manner on any immune 
      cell type. Hence, to identify cell types, the sole use of a small number of cell 
      surface markers in classical flow cytometry can be deceiving. Moreover, the 
      markers currently used to define mononuclear phagocyte subsets vary depending on 
      the tissue and animal species studied and even between laboratories. This has led 
      to confusion in the definition of DC subset identity and in their attribution of 
      specific functions. There is a strong need to identify a rigorous and consensus 
      way to define mononuclear phagocyte subsets, with precise guidelines potentially 
      applicable throughout tissues and species. We will discuss the advantages, 
      drawbacks, and complementarities of different methodologies: cell surface 
      phenotyping, ontogeny, functional characterization, and molecular profiling. We 
      will advocate that gene expression profiling is a very rigorous, largely unbiased 
      and accessible method to define the identity of mononuclear phagocyte subsets, 
      which strengthens and refines surface phenotyping. It is uniquely powerful to 
      yield new, experimentally testable, hypotheses on the ontogeny or functions of 
      mononuclear phagocyte subsets, their molecular regulation, and their evolutionary 
      conservation. We propose defining cell populations based on a combination of cell 
      surface phenotyping, expression analysis of hallmark genes, and robust functional 
      assays, in order to reach a consensus and integrate faster the huge but scattered 
      knowledge accumulated by different laboratories on different cell types, organs, 
      and species.
FAU - Vu Manh, Thien-Phong
AU  - Vu Manh TP
AD  - UM2, Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University , 
      Marseille , France ; U1104, Institut National de la Sante et de la Recherche 
      Medicale (INSERM) , Marseille , France ; UMR7280, Centre National de la Recherche 
      Scientifique (CNRS) , Marseille , France.
FAU - Bertho, Nicolas
AU  - Bertho N
AD  - Virologie et Immunologie Moleculaires UR892, Institut National de la Recherche 
      Agronomique , Jouy-en-Josas , France.
FAU - Hosmalin, Anne
AU  - Hosmalin A
AD  - INSERM U1016, Institut Cochin , Paris , France ; CNRS UMR8104 , Paris , France ; 
      Universite Paris Descartes , Paris , France ; Assistance Publique-Hopitaux de 
      Paris (AP-HP), Hopital Cochin , Paris , France.
FAU - Schwartz-Cornil, Isabelle
AU  - Schwartz-Cornil I
AD  - Virologie et Immunologie Moleculaires UR892, Institut National de la Recherche 
      Agronomique , Jouy-en-Josas , France.
FAU - Dalod, Marc
AU  - Dalod M
AD  - UM2, Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University , 
      Marseille , France ; U1104, Institut National de la Sante et de la Recherche 
      Medicale (INSERM) , Marseille , France ; UMR7280, Centre National de la Recherche 
      Scientifique (CNRS) , Marseille , France.
LA  - eng
GR  - 281225/ERC_/European Research Council/International
PT  - Journal Article
PT  - Review
DEP - 20150602
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC4451681
OTO - NOTNLM
OT  - chicken
OT  - comparative genomics
OT  - human
OT  - mononuclear phagocytes
OT  - mouse
OT  - non-human primates
OT  - pig
OT  - sheep
EDAT- 2015/06/18 06:00
MHDA- 2015/06/18 06:01
PMCR- 2015/01/01
CRDT- 2015/06/18 06:00
PHST- 2015/02/27 00:00 [received]
PHST- 2015/05/11 00:00 [accepted]
PHST- 2015/06/18 06:00 [entrez]
PHST- 2015/06/18 06:00 [pubmed]
PHST- 2015/06/18 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2015.00260 [doi]
PST - epublish
SO  - Front Immunol. 2015 Jun 2;6:260. doi: 10.3389/fimmu.2015.00260. eCollection 2015.