PMID- 26083946
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20181202
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 49
IP  - 14
DP  - 2015 Jul 21
TI  - Enhanced Deposition by Electrostatic Field-Assistance Aggravating Diesel Exhaust 
      Aerosol Toxicity for Human Lung Cells.
PG  - 8721-30
LID - 10.1021/acs.est.5b02503 [doi]
AB  - Air pollution is associated with increased risk of cardiovascular and pulmonary 
      diseases, but conventional air quality monitoring gives no information about 
      biological consequences. Exposing human lung cells at the air-liquid interface 
      (ALI) to ambient aerosol could help identify acute biological responses. This 
      study investigated electrode-assisted deposition of diesel exhaust aerosol (DEA) 
      on human lung epithelial cells (A549) in a prototype exposure chamber. A549 cells 
      were exposed to DEA at the ALI and under submerged conditions in different 
      electrostatic fields (EFs) and were assessed for cell viability, membrane 
      integrity, and IL-8 secretion. Qualitative differences of the DEA and its 
      deposition under different EFs were characterized using scanning mobility 
      particle sizer (SMPS) measurements, transmission electron microscopy (TEM), and 
      electron energy loss spectroscopy (EELS). Upon exposure to DEA only, cell 
      viability decreased and membrane impairment increased for cells at the ALI; 
      submerged cells were unaffected. These responses were enhanced upon application 
      of an EF, as was DEA deposition. No adverse effects were observed for filtered 
      DEA or air only, confirming particle-induced responses. The prototype exposure 
      chamber proved suitable for testing DEA-induced biological responses of cells at 
      the ALI using electrode-assisted deposition and may be useful for analysis of 
      other air pollutants.
FAU - Stoehr, Linda C
AU  - Stoehr LC
AD  - double daggerUniversity of Salzburg, Department of Molecular Biology, Hellbrunnerstrasse 34, 
      5020 Salzburg, Austria.
FAU - Madl, Pierre
AU  - Madl P
AD  - double daggerUniversity of Salzburg, Department of Molecular Biology, Hellbrunnerstrasse 34, 
      5020 Salzburg, Austria.
FAU - Boyles, Matthew S P
AU  - Boyles MS
AD  - double daggerUniversity of Salzburg, Department of Molecular Biology, Hellbrunnerstrasse 34, 
      5020 Salzburg, Austria.
FAU - Zauner, Roland
AU  - Zauner R
AD  - double daggerUniversity of Salzburg, Department of Molecular Biology, Hellbrunnerstrasse 34, 
      5020 Salzburg, Austria.
FAU - Wimmer, Monika
AU  - Wimmer M
AD  - double daggerUniversity of Salzburg, Department of Molecular Biology, Hellbrunnerstrasse 34, 
      5020 Salzburg, Austria.
FAU - Wiegand, Harald
AU  - Wiegand H
AD  -  section signKarlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
FAU - Andosch, Ancuela
AU  - Andosch A
AD  -  parallelUniversity of Salzburg, Department of Cell Biology, 5020 Salzburg, Austria.
FAU - Kasper, Gerhard
AU  - Kasper G
AD  -  section signKarlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
FAU - Pesch, Markus
AU  - Pesch M
FAU - Lutz-Meindl, Ursula
AU  - Lutz-Meindl U
AD  -  parallelUniversity of Salzburg, Department of Cell Biology, 5020 Salzburg, Austria.
FAU - Himly, Martin
AU  - Himly M
AD  - double daggerUniversity of Salzburg, Department of Molecular Biology, Hellbrunnerstrasse 34, 
      5020 Salzburg, Austria.
FAU - Duschl, Albert
AU  - Duschl A
AD  - double daggerUniversity of Salzburg, Department of Molecular Biology, Hellbrunnerstrasse 34, 
      5020 Salzburg, Austria.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150629
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Aerosols)
RN  - 0 (Air Pollutants)
RN  - 0 (Interleukin-8)
RN  - 0 (Vehicle Emissions)
SB  - IM
MH  - Aerosols/*toxicity
MH  - Air Pollutants/*toxicity
MH  - Air Pollution/analysis
MH  - Cell Line, Tumor
MH  - Cell Membrane/drug effects/metabolism
MH  - Cell Survival/drug effects
MH  - Epithelial Cells/*drug effects
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - Lung/drug effects/*pathology
MH  - Microscopy, Electron, Transmission
MH  - *Static Electricity
MH  - Vehicle Emissions/*analysis
EDAT- 2015/06/18 06:00
MHDA- 2016/02/24 06:00
CRDT- 2015/06/18 06:00
PHST- 2015/06/18 06:00 [entrez]
PHST- 2015/06/18 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
AID - 10.1021/acs.est.5b02503 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2015 Jul 21;49(14):8721-30. doi: 10.1021/acs.est.5b02503. 
      Epub 2015 Jun 29.