PMID- 26084029
OWN - NLM
STAT- MEDLINE
DCOM- 20150828
LR  - 20240210
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 42
IP  - 6
DP  - 2015 Jun 16
TI  - GM-CSF Mouse Bone Marrow Cultures Comprise a Heterogeneous Population of 
      CD11c(+)MHCII(+) Macrophages and Dendritic Cells.
PG  - 1197-211
LID - S1074-7613(15)00216-2 [pii]
LID - 10.1016/j.immuni.2015.05.018 [doi]
AB  - Dendritic cells (DCs) are key players in the immune system. Much of their biology 
      has been elucidated via culture systems in which hematopoietic precursors 
      differentiate into DCs under the aegis of cytokines. A widely used protocol 
      involves the culture of murine bone marrow (BM) cells with granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) to generate BM-derived DCs (BMDCs). BMDCs 
      express CD11c and MHC class II (MHCII) molecules and share with DCs isolated from 
      tissues the ability to present exogenous antigens to T cells and to respond to 
      microbial stimuli by undergoing maturation. We demonstrate that CD11c(+)MHCII(+) 
      BMDCs are in fact a heterogeneous group of cells that comprises conventional DCs 
      and monocyte-derived macrophages. DCs and macrophages in GM-CSF cultures both 
      undergo maturation upon stimulation with lipopolysaccharide but respond 
      differentially to the stimulus and remain separable entities. These results have 
      important implications for the interpretation of a vast array of data obtained 
      with DC culture systems.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Helft, Julie
AU  - Helft J
AD  - Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
FAU - Bottcher, Jan
AU  - Bottcher J
AD  - Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
FAU - Chakravarty, Probir
AU  - Chakravarty P
AD  - Bioinformatics Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
FAU - Zelenay, Santiago
AU  - Zelenay S
AD  - Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
FAU - Huotari, Jatta
AU  - Huotari J
AD  - Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
FAU - Schraml, Barbara U
AU  - Schraml BU
AD  - Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
FAU - Goubau, Delphine
AU  - Goubau D
AD  - Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
FAU - Reis e Sousa, Caetano
AU  - Reis e Sousa C
AD  - Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields 
      Laboratory, 44 Lincoln's Inn Fields, London WC2A 3LY, UK. Electronic address: 
      caetano@crick.ac.uk.
LA  - eng
SI  - GEO/GSE49358
SI  - GEO/GSE62361
SI  - GEO/GSE62746
GR  - 15689/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (CD11c Antigen)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lipopolysaccharides)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
CIN - Immunity. 2015 Jun 16;42(6):988-90. PMID: 26084019
CIN - Immunity. 2016 Jan 19;44(1):1-2. PMID: 26789912
CIN - Immunity. 2016 Jan 19;44(1):3-4. PMID: 26789913
CIN - Immunity. 2016 Jan 19;44(1):5-6. PMID: 26789914
MH  - Animals
MH  - Antigen Presentation
MH  - Bone Marrow Cells/*immunology
MH  - CD11c Antigen/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Immunophenotyping
MH  - Lipopolysaccharides/immunology
MH  - Lymphocyte Activation
MH  - Macrophages/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Transcriptome
EDAT- 2015/06/18 06:00
MHDA- 2015/09/01 06:00
CRDT- 2015/06/18 06:00
PHST- 2014/12/16 00:00 [received]
PHST- 2015/03/04 00:00 [revised]
PHST- 2015/03/31 00:00 [accepted]
PHST- 2015/06/18 06:00 [entrez]
PHST- 2015/06/18 06:00 [pubmed]
PHST- 2015/09/01 06:00 [medline]
AID - S1074-7613(15)00216-2 [pii]
AID - 10.1016/j.immuni.2015.05.018 [doi]
PST - ppublish
SO  - Immunity. 2015 Jun 16;42(6):1197-211. doi: 10.1016/j.immuni.2015.05.018.