PMID- 26084202
OWN - NLM
STAT- MEDLINE
DCOM- 20160503
LR  - 20220408
IS  - 1365-2443 (Electronic)
IS  - 1356-9597 (Linking)
VI  - 20
IP  - 8
DP  - 2015 Aug
TI  - Loss of ADAR1 in human iPS cells promotes caspase3-mediated apoptotic cell death.
PG  - 675-80
LID - 10.1111/gtc.12261 [doi]
AB  - Adenosine deaminases acting on RNA (ADARs) convert adenosine residues to inosine 
      specifically in double-stranded RNAs. In this study, we investigated the function 
      of primary RNA editing enzyme ADAR1 in pluripotent stem cells and found that loss 
      of ADAR1 in human iPS cells promotes caspase3-mediated cell death. However, ADAR1 
      knockdown (KD) did not alter cell morphology, alkaline phosphatase (AP) staining 
      activities and the expression levels of pluripotent marker genes, indicating that 
      ADAR1 is dispensable for maintenance of pluripotency. Furthermore, ADAR1-KD iPS 
      cells did not change proliferation rate. These findings extended the role of 
      ADAR1 and might open the road for understanding pluripotent state more deeply.
CI  - (c) 2015 The Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.
FAU - Katayama, Shota
AU  - Katayama S
AD  - Animal Stem Cell Project, Animal Reproduction Unit, Hokkaido Center for Animal 
      Science, Shinseiminami, Memuro-cho, Hokkaido, 082-0081, Japan.
FAU - Shimoda, Kaori
AU  - Shimoda K
AD  - Animal Stem Cell Project, Animal Reproduction Unit, Hokkaido Center for Animal 
      Science, Shinseiminami, Memuro-cho, Hokkaido, 082-0081, Japan.
FAU - Takenaga, Yuji
AU  - Takenaga Y
AD  - Animal Stem Cell Project, Animal Reproduction Unit, Hokkaido Center for Animal 
      Science, Shinseiminami, Memuro-cho, Hokkaido, 082-0081, Japan.
LA  - eng
PT  - Journal Article
DEP - 20150618
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (RNA-Binding Proteins)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.5.4.37 (ADAR protein, human)
RN  - EC 3.5.4.4 (Adenosine Deaminase)
SB  - IM
MH  - Adenosine Deaminase/*genetics/metabolism
MH  - *Apoptosis
MH  - Caspase 3/*metabolism
MH  - Cell Line
MH  - Humans
MH  - Induced Pluripotent Stem Cells/cytology/*metabolism
MH  - RNA-Binding Proteins/*genetics/metabolism
EDAT- 2015/06/19 06:00
MHDA- 2016/05/04 06:00
CRDT- 2015/06/19 06:00
PHST- 2015/04/16 00:00 [received]
PHST- 2015/05/13 00:00 [accepted]
PHST- 2015/06/19 06:00 [entrez]
PHST- 2015/06/19 06:00 [pubmed]
PHST- 2016/05/04 06:00 [medline]
AID - 10.1111/gtc.12261 [doi]
PST - ppublish
SO  - Genes Cells. 2015 Aug;20(8):675-80. doi: 10.1111/gtc.12261. Epub 2015 Jun 18.