PMID- 26084614
OWN - NLM
STAT- MEDLINE
DCOM- 20160329
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 36
IP  - 11
DP  - 2015 Nov
TI  - Lip cancer and pre-cancerous lesions harbor TP53 mutations, exhibit allelic loss 
      at 9p, 9q, and 17p, but no BRAFV600E mutations.
PG  - 9059-66
LID - 10.1007/s13277-015-3659-9 [doi]
AB  - Molecular mechanisms of lip squamous cell carcinoma (LSCC) and actinic cheilitis 
      (AC) are unclear. We aimed at assessing loss of heterozygosity (LOH) and TP53 and 
      BRAF V600E mutations in these lesions. Formalin-fixed paraffin-embedded (FFPE) 
      samples of 17 LSCC and 16 AC were included, with additional 5 fresh LSCC 
      genotyped for TP53 mutations. LOH was assessed by six polymorphic markers located 
      at 9p22, 9q22, and 17p13 and correlated with cell proliferation (Ki-67) and P53 
      immunostaining. Direct sequencing of TP53 exons 2-11 (fresh samples), and exons 
      5-9 (FFPE samples) was carried out. BRAF V600E mutation was genotyped in eight 
      LSCC. LOH occurred in at least one marker in 15/17 LSCC and in 9/16 AC. The 
      marker exhibiting the highest frequency of allelic loss (FAL) in LSCC was D9S157 
      (8/12 informative cases) and D9S287 in AC (4/11 informative cases). Cell 
      proliferation was not correlated with LOH or with the FAL and no correlation 
      between P53 IHC and 17p LOH was observed. We found TP53 missense mutations in 
      both lesions and nonsense in LSCC, including CC>TT transition, which is a marker 
      of UV damage. BRAF V600E mutation was not detected. LOH and TP53 mutations 
      detected in LSCC and AC may be associated with tumorigenesis, whereas BRAF V600E 
      mutation does not seem to significantly contribute to LSCC pathogenesis.
FAU - Correa, Gefter Thiago Batista
AU  - Correa GT
AD  - Department of Oral Surgery and Pathology, School of Dentistry, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Bernardes, Vanessa Fatima
AU  - Bernardes VF
AD  - Department of Pathology, Biological Sciences Institute, Universidade Federal de 
      Minas Gerais, Av. Antonio Carlos 6627, Pampulha, Belo Horizonte, Minas Gerais, 
      31270-901, Brazil.
FAU - de Sousa, Silvia Ferreira
AU  - de Sousa SF
AD  - Department of Oral Surgery and Pathology, School of Dentistry, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Diniz, Marina Goncalves
AU  - Diniz MG
AD  - Department of Oral Surgery and Pathology, School of Dentistry, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Salles, Jose Maria Porcaro
AU  - Salles JM
AD  - Department of Surgery, School of Medicine, Universidade Federal de Minas Gerais, 
      Belo Horizonte, Brazil.
FAU - Souza, Renan Pedra
AU  - Souza RP
AD  - Department of General Biology, Biological Sciences Institute, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - De-Paula, Alfredo Mauricio Batista
AU  - De-Paula AM
AD  - Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, 
      Minas Gerais, Brazil.
FAU - Gomez, Ricardo Santiago
AU  - Gomez RS
AD  - Department of Oral Surgery and Pathology, School of Dentistry, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Gomes, Carolina Cavalieri
AU  - Gomes CC
AD  - Department of Pathology, Biological Sciences Institute, Universidade Federal de 
      Minas Gerais, Av. Antonio Carlos 6627, Pampulha, Belo Horizonte, Minas Gerais, 
      31270-901, Brazil. carolinacgomes@ufmg.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150618
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (TP53 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.11.1 (BRAF protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf)
RN  - Actinic cheilitis
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Proliferation/*genetics
MH  - Cheilitis/genetics/pathology
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Exons
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - Lip Neoplasms/*genetics/pathology
MH  - Loss of Heterozygosity
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Proto-Oncogene Proteins B-raf/*genetics
MH  - Tumor Suppressor Protein p53/biosynthesis/*genetics
OTO - NOTNLM
OT  - Actinic cheilitis
OT  - Head and neck cancer
OT  - LOH
OT  - Lip squamous cell carcinoma
OT  - Potentially malignant oral lesions
OT  - p53
EDAT- 2015/06/19 06:00
MHDA- 2016/03/30 06:00
CRDT- 2015/06/19 06:00
PHST- 2015/04/27 00:00 [received]
PHST- 2015/06/09 00:00 [accepted]
PHST- 2015/06/19 06:00 [entrez]
PHST- 2015/06/19 06:00 [pubmed]
PHST- 2016/03/30 06:00 [medline]
AID - 10.1007/s13277-015-3659-9 [pii]
AID - 10.1007/s13277-015-3659-9 [doi]
PST - ppublish
SO  - Tumour Biol. 2015 Nov;36(11):9059-66. doi: 10.1007/s13277-015-3659-9. Epub 2015 
      Jun 18.