PMID- 26085990
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150618
LR  - 20200930
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 8
IP  - 3
DP  - 2015
TI  - Anthrax lethal toxin suppresses high glucose induced VEGF over secretion through 
      a post-translational mechanism.
PG  - 453-8
LID - 10.3980/j.issn.2222-3959.2015.03.04 [doi]
AB  - AIM: To prove anthrax lethal toxin (LeTx) blocks the mitogen activated protein 
      kinases (MAPKs) activation by degrading the MAPK/ERK kinases (MEKs) to suppress 
      vascular endothelial growth factor (VEGF) secretion. METHODS: Human adult retinal 
      pigmented epithelium (ARPE) cells were cultured and treated with normal glucose, 
      high glucose or high glucose with LeTx for additional 24, 48 or 72h for viable 
      cell count. Total RNA from the ARPE was isolated for reverse transcription 
      polymerase chain reaction (RT-PCR). The conditioned medium of ARPE cells treated 
      in different group for 48h was filtered and diluted to detect the concentration 
      of VEGF by enzyme-linked immunosorbant assays. Evaluate the role of MEK/MAPK 
      pathway in the secretion of VEGF by immunoblotting. RESULTS: In this study, we 
      proved high glucose induced activation of the MAPK extracellular signal-regulated 
      kinase (ERK1/2) and p38 in the ARPE cell line was blocked by anthrax LeTx. LeTx 
      also inhibited high glucose induced ARPE cell over proliferation. CONCLUSION: 
      LeTx suppressed high glucose induced VEGF over secretion in the ARPE cells, 
      mainly through a post-translational mechanism.
FAU - Zhang, Wei-Wei
AU  - Zhang WW
AD  - Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, Jiangsu Province, China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Endocrinology, Jiangsu Province Hospital of TCM, Nanjing 210029, 
      Jiangsu Province, China.
FAU - Xie, Ping
AU  - Xie P
AD  - Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, Jiangsu Province, China.
FAU - Yuan, Song-Tao
AU  - Yuan ST
AD  - Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, Jiangsu Province, China.
FAU - Liu, Qing-Huai
AU  - Liu QH
AD  - Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, Jiangsu Province, China.
LA  - eng
PT  - Journal Article
DEP - 20150618
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC4458645
OTO - NOTNLM
OT  - angiogenesis
OT  - anthrax lethal toxin
OT  - diabetic retinopathy
OT  - retinal pigmented epithelium
OT  - vascular endothelial growth factor
EDAT- 2015/06/19 06:00
MHDA- 2015/06/19 06:01
PMCR- 2015/06/18
CRDT- 2015/06/19 06:00
PHST- 2014/08/16 00:00 [received]
PHST- 2014/12/29 00:00 [accepted]
PHST- 2015/06/19 06:00 [entrez]
PHST- 2015/06/19 06:00 [pubmed]
PHST- 2015/06/19 06:01 [medline]
PHST- 2015/06/18 00:00 [pmc-release]
AID - ijo-08-03-453 [pii]
AID - 10.3980/j.issn.2222-3959.2015.03.04 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2015 Jun 18;8(3):453-8. doi: 
      10.3980/j.issn.2222-3959.2015.03.04. eCollection 2015.