PMID- 26086561
OWN - NLM
STAT- MEDLINE
DCOM- 20160527
LR  - 20221207
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 141
DP  - 2015 Dec
TI  - The fate of a nitrobenzene-degrading bacterium in pharmaceutical wastewater 
      treatment sludge.
PG  - 13-8
LID - S0045-6535(15)00585-8 [pii]
LID - 10.1016/j.chemosphere.2015.05.098 [doi]
AB  - This paper describes the fate of a nitrobenzene-degrading bacterium, Klebsiella 
      oxytoca NBA-1, which was isolated from a pharmaceutical wastewater treatment 
      facility. The 90-day survivability of strain NBA-1 after exposure to sludge under 
      anaerobic and aerobic conditions was investigated. The bacterium was inoculated 
      into sludge amended with glucose and p-chloronitrobenzene (p-CNB) to compare the 
      bacterial community variations between the modified sludge and nitrobenzene 
      amendment. The results showed that glucose had no obvious effect on nitrobenzene 
      biodegradation in the co-metabolism process, regardless of the presence/absence 
      of oxygen. When p-CNB was added under anaerobic conditions, the biodegradation 
      rate of nitrobenzene remained unchanged although p-CNB inhibited the production 
      of aniline. The diversity of the microbial community increased and NBA-1 
      continued to be one of the dominant strains. Under aerobic conditions, the 
      degradation rate of both nitrobenzene and p-CNB was only 20% of that under 
      anaerobic conditions. p-CNB had a toxic effect on the microorganisms in the 
      sludge so that most of the DGGE (denaturing gradient gel electrophoresis) bands, 
      including that of NBA-1, began to disappear under aerobic conditions after 90days 
      of exposure. These data show that the bacterial community was stable under 
      anaerobic conditions and the microorganisms, including NBA-1, were more resistant 
      to the adverse environment.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Ren, Yuan
AU  - Ren Y
AD  - School of Environment and Energy, South China University of Technology, Higher 
      Education Mega Center, Panyu District, Guangzhou 510006, PR China; The Key 
      Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, 
      Ministry of Education, PR China; The Key Laboratory of Environmental Protection 
      and Eco-Remediation of Guangdong Regular Higher Education Institutions, PR China. 
      Electronic address: ceyren@scut.edu.cn.
FAU - Yang, Juan
AU  - Yang J
AD  - School of Environment and Energy, South China University of Technology, Higher 
      Education Mega Center, Panyu District, Guangzhou 510006, PR China.
FAU - Chen, Shaoyi
AU  - Chen S
AD  - School of Environment and Energy, South China University of Technology, Higher 
      Education Mega Center, Panyu District, Guangzhou 510006, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150615
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Nitrobenzenes)
RN  - 0 (Sewage)
RN  - 0 (Waste Water)
RN  - 0 (Water Pollutants, Chemical)
RN  - D1YI9R2K8O (2-chloronitrobenzene)
RN  - E57JCN6SSY (nitrobenzene)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Klebsiella oxytoca/*growth & development
MH  - Nitrobenzenes/*analysis
MH  - *Sewage/chemistry/microbiology
MH  - *Wastewater/chemistry/microbiology
MH  - Water Pollutants, Chemical/*analysis
MH  - Water Purification/*methods
OTO - NOTNLM
OT  - Bacterial community
OT  - Fate
OT  - Nitrobenzene-degrading bacterium
OT  - PCR-DGGE
OT  - Pharmaceutical sludge
EDAT- 2015/06/19 06:00
MHDA- 2016/05/28 06:00
CRDT- 2015/06/19 06:00
PHST- 2014/12/29 00:00 [received]
PHST- 2015/05/09 00:00 [revised]
PHST- 2015/05/29 00:00 [accepted]
PHST- 2015/06/19 06:00 [entrez]
PHST- 2015/06/19 06:00 [pubmed]
PHST- 2016/05/28 06:00 [medline]
AID - S0045-6535(15)00585-8 [pii]
AID - 10.1016/j.chemosphere.2015.05.098 [doi]
PST - ppublish
SO  - Chemosphere. 2015 Dec;141:13-8. doi: 10.1016/j.chemosphere.2015.05.098. Epub 2015 
      Jun 15.