PMID- 26086806
OWN - NLM
STAT- MEDLINE
DCOM- 20160212
LR  - 20240324
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 87
IP  - 14
DP  - 2015 Jul 21
TI  - Definitive Screening Design Optimization of Mass Spectrometry Parameters for 
      Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT 
      Plasma N-Glycans.
PG  - 7305-12
LID - 10.1021/acs.analchem.5b01609 [doi]
AB  - High-throughput, quantitative processing of N-linked glycans would facilitate 
      large-scale studies correlating the glycome with disease and open the field to 
      basic and applied researchers. We sought to meet these goals by coupling 
      filter-aided-N-glycan separation (FANGS) to the individuality normalization when 
      labeling with glycan hydrazide tags (INLIGHT) for analysis of plasma. A 
      quantitative comparison of this method was conducted against solid phase 
      extraction (SPE), a ubiquitous and trusted method for glycan purification. We 
      demonstrate that FANGS-INLIGHT purification was not significantly different from 
      SPE in terms of glycan abundances, variability, functional classes, or molecular 
      weight distributions. Furthermore, to increase the depth of glycome coverage, we 
      executed a definitive screening design of experiments (DOE) to optimize the MS 
      parameters for glycan analyses. We optimized MS parameters across five N-glycan 
      responses using a standard glycan mixture, translated these to plasma and 
      achieved up to a 3-fold increase in ion abundances.
FAU - Hecht, Elizabeth S
AU  - Hecht ES
AD  - Department of Chemistry, North Carolina State University, Raleigh, North Carolina 
      27695, United States.
FAU - McCord, James P
AU  - McCord JP
AD  - Department of Chemistry, North Carolina State University, Raleigh, North Carolina 
      27695, United States.
FAU - Muddiman, David C
AU  - Muddiman DC
AD  - Department of Chemistry, North Carolina State University, Raleigh, North Carolina 
      27695, United States.
LA  - eng
GR  - R33 CA147988/CA/NCI NIH HHS/United States
GR  - T32 GM008776/GM/NIGMS NIH HHS/United States
GR  - CA147988-02/CA/NCI NIH HHS/United States
GR  - T32GM008776/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150630
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Equipment Design
MH  - Mass Spectrometry/instrumentation
MH  - Polysaccharides/blood/*isolation & purification
MH  - *Solid Phase Extraction/instrumentation
PMC - PMC4664066
MID - NIHMS739512
EDAT- 2015/06/19 06:00
MHDA- 2016/02/13 06:00
PMCR- 2016/07/21
CRDT- 2015/06/19 06:00
PHST- 2015/06/19 06:00 [entrez]
PHST- 2015/06/19 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
PHST- 2016/07/21 00:00 [pmc-release]
AID - 10.1021/acs.analchem.5b01609 [doi]
PST - ppublish
SO  - Anal Chem. 2015 Jul 21;87(14):7305-12. doi: 10.1021/acs.analchem.5b01609. Epub 
      2015 Jun 30.