PMID- 26091522
OWN - NLM
STAT- MEDLINE
DCOM- 20160413
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 6
DP  - 2015
TI  - scFv from Antibody That Mimics gp43 Modulates the Cellular and Humoral Immune 
      Responses during Experimental Paracoccidioidomycosis.
PG  - e0129401
LID - 10.1371/journal.pone.0129401 [doi]
LID - e0129401
AB  - Paracoccidioidomycosis (PCM), caused by Paracoccidioides species is a prevalent 
      systemic and progressive mycosis that occurs in Latin America. It is caused by 
      Paracoccidioides species. Immunization with dendritic cells transfected with a 
      plasmid encoding the scFv (pMAC/PS-scFv) that mimics the main antigen of P. 
      brasiliensis (gp43) confers protection in experimental PCM. DCs link innate and 
      adaptive immunity by recognizing invading pathogens and selecting the type of 
      effector T cell to mediate the immune response. Here, we showed that 
      DC-pMAC/PS-scFv induces the activation of CD4+ and CD8+ T cells. Moreover, our 
      results demonstrated that BALB/c mice infected with P. brasiliensis and treated 
      with DC-pMAC/PS-scFv showed the induction of specific IgG production against gp43 
      and IFN-gamma, IL-12 and IL-4 cytokines. Analysis of regional lymph nodes revealed 
      increases in the expression of clec7a, myd88, tlr2, gata3 and tbx21, which are 
      involved in the immune response. Taken together, our results indicate that the 
      scFv modulates the humoral and cellular immune responses and presents epitopes to 
      CD4+ and CD8+ T cells.
FAU - Jannuzzi, Grasielle Pereira
AU  - Jannuzzi GP
AD  - Departamento de Analises Clinicas, Faculdade de Ciencias Farmaceuticas da 
      Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Tavares, Aldo Henrique F P
AU  - Tavares AH
AD  - Instituto de Biologia, Universidade de Brasilia, Brasilia, Brazil.
FAU - Kaihami, Gilberto Hideo
AU  - Kaihami GH
AD  - Departamento de Quimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, Brazil.
FAU - de Almeida, Jose Roberto Fogaca
AU  - de Almeida JR
AD  - Departamento de Analises Clinicas, Faculdade de Ciencias Farmaceuticas da 
      Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - de Almeida, Sandro Rogerio
AU  - de Almeida SR
AD  - Departamento de Analises Clinicas, Faculdade de Ciencias Farmaceuticas da 
      Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Ferreira, Karen Spadari
AU  - Ferreira KS
AD  - Departamento de Ciencias Biologicas do Instituto de Ciencias Ambientais, Quimicas 
      e Farmaceuticas, Universidade Federal de Sao Paulo, Diadema, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150619
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Single-Chain Antibodies)
SB  - IM
MH  - Animals
MH  - Antigens, Fungal/*immunology
MH  - Chemotaxis, Leukocyte/immunology
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/immunology/metabolism
MH  - Disease Models, Animal
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Female
MH  - Fungal Proteins/*immunology
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Glycoproteins/*immunology
MH  - *Immunity, Cellular
MH  - *Immunity, Humoral
MH  - Immunization
MH  - Immunotherapy, Adoptive
MH  - Lymph Nodes/immunology/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Count
MH  - Mice
MH  - Paracoccidioidomycosis/genetics/*immunology/metabolism/therapy
MH  - Single-Chain Antibodies/genetics/*immunology
MH  - T-Lymphocyte Subsets/immunology/metabolism
MH  - Transfection
PMC - PMC4474724
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/06/20 06:00
MHDA- 2016/04/14 06:00
PMCR- 2015/06/19
CRDT- 2015/06/20 06:00
PHST- 2014/08/19 00:00 [received]
PHST- 2015/05/07 00:00 [accepted]
PHST- 2015/06/20 06:00 [entrez]
PHST- 2015/06/20 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
PHST- 2015/06/19 00:00 [pmc-release]
AID - PONE-D-14-33037 [pii]
AID - 10.1371/journal.pone.0129401 [doi]
PST - epublish
SO  - PLoS One. 2015 Jun 19;10(6):e0129401. doi: 10.1371/journal.pone.0129401. 
      eCollection 2015.