PMID- 26093497 OWN - NLM STAT- MEDLINE DCOM- 20151208 LR - 20210105 IS - 1096-0341 (Electronic) IS - 0042-6822 (Linking) VI - 484 DP - 2015 Oct TI - Glycoprotein 5 of porcine reproductive and respiratory syndrome virus strain SD16 inhibits viral replication and causes G2/M cell cycle arrest, but does not induce cellular apoptosis in Marc-145 cells. PG - 136-145 LID - S0042-6822(15)00275-5 [pii] LID - 10.1016/j.virol.2015.05.019 [doi] AB - Cell apoptosis is common after infection with porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV GP5 has been reported to induce cell apoptosis. To further understand the role of GP5 in PRRSV induced cell apoptosis, we established Marc-145 cell lines stably expressing full-length GP5, GP5(Delta84-96) (aa 84-96 deletion), and GP5(Delta97-119) (aa 97-119 deletion). Cell proliferation, cell cycle progression, cell apoptosis and virus replication in these cell lines were evaluated. Neither truncated nor full-length GP5 induced cell apoptosis in Marc-145 cells. However, GP5(Delta97-119), but not full-length or GP5(Delta84-96), induced a cell cycle arrest at the G2/M phase resulting in a reduction in the growth of Marc-145 cells. Additionally, GP5(Delta84-96) inhibited the replication of PRRSV in Marc-145 cells through induction of IFN-beta. These findings suggest that PRRSV GP5 is not responsible for inducing cell apoptosis in Marc-145 cells under these experimental conditions; however it has other important roles in virus/host cell biology. CI - Copyright (c) 2015 Elsevier Inc. All rights reserved. FAU - Mu, Yang AU - Mu Y AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: muyang@nwsuaf.edu.cn. FAU - Li, Liangliang AU - Li L AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: lifeiyang2007@126.com. FAU - Zhang, Beibei AU - Zhang B AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: diana851218@163.com. FAU - Huang, Baicheng AU - Huang B AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: hbch228@163.com. FAU - Gao, Jiming AU - Gao J AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: jimingao2006@163.com. FAU - Wang, Xiangpeng AU - Wang X AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: wangxiangpeng2003@126.com. FAU - Wang, Chengbao AU - Wang C AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: wangchengbao@126.com. FAU - Xiao, Shuqi AU - Xiao S AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: shqxiaojd@hotmail.com. FAU - Zhao, Qin AU - Zhao Q AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: qinzhao_2004@sina.com. FAU - Sun, Yani AU - Sun Y AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: yanisun_2005@sina.com. FAU - Zhang, Gaiping AU - Zhang G AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China. Electronic address: Zhanggaiping2003@yahoo.com.cn. FAU - Hiscox, Julian A AU - Hiscox JA AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK. Electronic address: julian.hiscox@liverpool.ac.uk. FAU - Zhou, En-Min AU - Zhou EM AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: zhouem@nwsuaf.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150619 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Viral Envelope Proteins) RN - 0 (glycoprotein 5, PRRSV) SB - IM MH - Animals MH - *Apoptosis MH - *Cell Cycle Checkpoints MH - Cell Line MH - Cell Proliferation MH - Gene Deletion MH - *Host-Pathogen Interactions MH - Porcine respiratory and reproductive syndrome virus/genetics/*physiology MH - Swine MH - Viral Envelope Proteins/*metabolism MH - *Virus Replication OTO - NOTNLM OT - Cell apoptosis OT - GP5 OT - IFN OT - PRRSV OT - Virus replication EDAT- 2015/06/22 06:00 MHDA- 2015/12/15 06:00 CRDT- 2015/06/22 06:00 PHST- 2014/10/31 00:00 [received] PHST- 2015/03/23 00:00 [revised] PHST- 2015/05/30 00:00 [accepted] PHST- 2015/06/22 06:00 [entrez] PHST- 2015/06/22 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] AID - S0042-6822(15)00275-5 [pii] AID - 10.1016/j.virol.2015.05.019 [doi] PST - ppublish SO - Virology. 2015 Oct;484:136-145. doi: 10.1016/j.virol.2015.05.019. Epub 2015 Jun 19.