PMID- 26093609
OWN - NLM
STAT- MEDLINE
DCOM- 20151221
LR  - 20220331
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 128
IP  - 9
DP  - 2015 Sep
TI  - Introgression of Agropyron cristatum 6P chromosome segment into common wheat for 
      enhanced thousand-grain weight and spike length.
PG  - 1827-37
LID - 10.1007/s00122-015-2550-9 [doi]
AB  - This study explored the genetic constitutions of wheat-Agropyron cristatum 6P 
      chromosomal translocation and determined the effects of 6P intercalary chromosome 
      segment on thousand-grain weight and spike length in wheat. Crop wild relatives 
      provide rich genetic resources for wheat improvement. Introduction of alien genes 
      from Agropyron cristatum into common wheat can broaden its genetic diversity. In 
      this study, radiation-induced wheat-A. cristatum translocation line Pubing3035 
      derived from the offspring of wheat-A. cristatum 6P chromosomes addition line was 
      identified and analyzed using genomic in situ hybridization (GISH), dual-color 
      fluorescence in situ hybridization (FISH), and molecular markers. GISH analysis 
      revealed that Pubing3035 was a Ti1AS-6PL-1AS.1AL intercalary translocation. The 
      breakpoint was pinpointed to locate near the centromeric region on the short arm 
      of wheat chromosome 1A based on a constructed F2 linkage map and it was flanked 
      by markers SSR12 and SSR263. The genotypic data, combined with the phenotypes, 
      indicated that A. cristatum 6P chromosomal segment played an important role in 
      regulating the thousand-grain weight and spike length. On average, the 
      thousand-grain weight and spike length in translocation individuals were 
      approximately 2.5 g higher and 0.7 cm longer than those in non-translocation 
      individuals in F2 and BC1F1 populations. The clusters of quantitative trait loci 
      for thousand-grain weight, spike length, and spikelet density contributed by 6P 
      chromosome segment were mapped between A. cristatum unique marker Agc7155 and 
      wheat marker SSR263, which, respectively, explained phenotypic variance of 24.96, 
      12.38 and 17.20 % with an LOD of 10.63, 4.89 and 5.59. Overall, the translocation 
      Pubing3035 had a positive effect on the yield of wheat, which laid the foundation 
      for the localization of A. cristatum excellent genes and made itself a promising 
      and valuable germplasm for wheat improvement.
FAU - Zhang, Jing
AU  - Zhang J
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, 
      China.
FAU - Zhang, Jinpeng
AU  - Zhang J
FAU - Liu, Weihua
AU  - Liu W
FAU - Han, Haiming
AU  - Han H
FAU - Lu, Yuqing
AU  - Lu Y
FAU - Yang, Xinming
AU  - Yang X
FAU - Li, Xiuquan
AU  - Li X
FAU - Li, Lihui
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150621
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Agropyron/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Plant
MH  - Edible Grain/genetics
MH  - Expressed Sequence Tags
MH  - Genetic Linkage
MH  - Genetic Markers
MH  - Genotype
MH  - *Hybridization, Genetic
MH  - Quantitative Trait Loci
MH  - Seeds/*growth & development
MH  - *Translocation, Genetic
MH  - Triticum/*genetics
EDAT- 2015/06/22 06:00
MHDA- 2015/12/22 06:00
CRDT- 2015/06/22 06:00
PHST- 2015/04/02 00:00 [received]
PHST- 2015/05/29 00:00 [accepted]
PHST- 2015/06/22 06:00 [entrez]
PHST- 2015/06/22 06:00 [pubmed]
PHST- 2015/12/22 06:00 [medline]
AID - 10.1007/s00122-015-2550-9 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2015 Sep;128(9):1827-37. doi: 10.1007/s00122-015-2550-9. Epub 
      2015 Jun 21.