PMID- 26094210
OWN - NLM
STAT- MEDLINE
DCOM- 20160113
LR  - 20150708
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 997
DP  - 2015 Aug 1
TI  - Application of GC/MS-based metabonomic profiling in studying the therapeutic 
      effects of Huangbai-Zhimu herb-pair (HZ) extract on streptozotocin-induced type 2 
      diabetes in mice.
PG  - 96-104
LID - S1570-0232(15)30002-7 [pii]
LID - 10.1016/j.jchromb.2015.05.003 [doi]
AB  - A protocol for metabolic profiling of mice urine was developed based on gas 
      chromatograph-mass spectrometer (GC-MS) to explore metabolic state directly. The 
      intra-day, inter-day, repeatability, and stability RSD for most endogenous 
      compounds were less than 3%. Type 2 diabetic mellitus (T2DM) mice model was 
      induced by high calorie diet combined with streptozocin. Urine from the control, 
      T2DM and Huangbai-Zhimu herb-pair (HZ) treatment mice were enrolled in the 
      subsequent study to show the usefulness of the method. OPLS-DA scores plots 
      demonstrate that the cluster of T2DM mice is separated from that of control mice, 
      while HZ-T2DM mice are located close to control mice, indicating that metabolic 
      profiles of these HZ-T2DM mice are placed toward those of control group. The 
      results illustrate that HZ treatment could lower the level of d-glucose, 
      hexadecanoic acid, octadecanoic acid, propanoic acid, 3-hydroxybutyric acid, and 
      2,3-dihydroxybutanoic acid in urine of DM mice, meanwhile the results show that 
      HZ treatment could ameliorate T2DM symptoms by intervening the fatty acid 
      metabolism, starch and sucrose metabolism, and glyoxylate and dicarboxylate 
      metabolism. This preliminary application indicated that the method is suitable 
      and reliable for urine metabolic profiling. This study might explain the 
      metabolic effects of T2DM and the mechanisms of action of HZ against T2DM.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Song, Lili
AU  - Song L
AD  - Department of Experimental Teaching, Tianjin University of Traditional Chinese 
      Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China.
FAU - Liu, Hongyue
AU  - Liu H
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China.
FAU - Wang, Yan
AU  - Wang Y
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China.
FAU - Wang, Yuming
AU  - Wang Y
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China.
FAU - Liu, Jinbiao
AU  - Liu J
AD  - School of Chemistry & Chemical Engineering, Tianjin University of Technology, 391 
      Binshui West Street, Xiqing District, Tianjin 300384, People's Republic of China.
FAU - Zhou, Zhensheng
AU  - Zhou Z
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China.
FAU - Chu, Huilun
AU  - Chu H
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China.
FAU - Zhuang, Pengwei
AU  - Zhuang P
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China; Tianjin JF-Pharmaland Technology Development Co., Ltd., 
      Tianjin, China. Electronic address: zhuangpengwei@163.com.
FAU - Zhang, Yanjun
AU  - Zhang Y
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's 
      Republic of China. Electronic address: 13820188818@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150518
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Blood Glucose)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Fatty Acids)
RN  - 0 (Saponins)
RN  - 0 (huangbai)
RN  - 0 (zhi-mu saponin)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Blood Glucose/drug effects
MH  - Body Weight/drug effects
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Fatty Acids/metabolism
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Male
MH  - Metabolome/*drug effects
MH  - Metabolomics/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Reproducibility of Results
MH  - Saponins/*pharmacology
MH  - Streptozocin
OTO - NOTNLM
OT  - GC-MS
OT  - Huangbai-Zhimu herb-pair
OT  - Type 2 diabetes
OT  - Urine metabonomics
EDAT- 2015/06/22 06:00
MHDA- 2016/01/14 06:00
CRDT- 2015/06/22 06:00
PHST- 2014/11/11 00:00 [received]
PHST- 2015/04/24 00:00 [revised]
PHST- 2015/05/07 00:00 [accepted]
PHST- 2015/06/22 06:00 [entrez]
PHST- 2015/06/22 06:00 [pubmed]
PHST- 2016/01/14 06:00 [medline]
AID - S1570-0232(15)30002-7 [pii]
AID - 10.1016/j.jchromb.2015.05.003 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Aug 1;997:96-104. doi: 
      10.1016/j.jchromb.2015.05.003. Epub 2015 May 18.