PMID- 26095306
OWN - NLM
STAT- MEDLINE
DCOM- 20160627
LR  - 20150929
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 36
IP  - 19
DP  - 2015 Oct
TI  - Aptamer-based detection and quantitative analysis of human immunoglobulin E in 
      capillary electrophoresis with chemiluminescence detection.
PG  - 2413-8
LID - 10.1002/elps.201500158 [doi]
AB  - A novel aptamer-based CE with chemiluminescence (CL) assay was developed for 
      highly sensitive detection of human immunoglobulin E (IgE). The IgE aptamer was 
      conjugated with gold nanoparticles (AuNPs) to form AuNPs-aptamer that could 
      specifically recognize the IgE to produce an AuNPs-aptamer-IgE complex. The 
      mixture of the AuNPs-aptamer-IgE complex and the unbounded AuNPs-aptamer could be 
      effectively separated by CE and sensitively detected with luminol-H2 O2 CL 
      system. By taking the advantage of the excellent catalytic behavior of AuNPs on 
      luminol-H2 O2 CL system, the ultrasensitive detection of IgE was achieved. The 
      detection limit of IgE is 7.6 fM (S/N = 3) with a linear range from 0.025 to 250 
      pM. Successful detection of IgE in human serum samples was demonstrated and the 
      recoveries of 94.9-103.2% were obtained. The excellent assay features of the 
      developed approach are its specificity, sensitivity, adaptability, and very small 
      sample consumption. Our design provides a methodology model for determination of 
      rare proteins in biological samples.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Liu, Yan-Ming
AU  - Liu YM
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, P. R. China.
FAU - Cao, Jun-Tao
AU  - Cao JT
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, P. R. China.
FAU - Liu, Ying-Ying
AU  - Liu YY
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, P. R. China.
FAU - Zhang, Jing-Jing
AU  - Zhang JJ
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, P. R. China.
FAU - Zhou, Min
AU  - Zhou M
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, P. R. China.
FAU - Huang, Ke-Jing
AU  - Huang KJ
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, P. R. China.
FAU - Chen, Yong-Hong
AU  - Chen YH
AD  - Xinyang Central Hospital, Xinyang, P. R. China.
FAU - Ren, Shu-Wei
AU  - Ren SW
AD  - Xinyang Central Hospital, Xinyang, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150729
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Gold/chemistry
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Limit of Detection
MH  - Linear Models
MH  - Luminescent Measurements/*methods
MH  - Metal Nanoparticles/chemistry
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Aptamer
OT  - Capillary electrophoresis
OT  - Chemiluminescence
OT  - Gold nanoparticles
OT  - Human immunoglobulin E
EDAT- 2015/06/23 06:00
MHDA- 2016/06/28 06:00
CRDT- 2015/06/23 06:00
PHST- 2015/03/24 00:00 [received]
PHST- 2015/04/27 00:00 [revised]
PHST- 2015/05/18 00:00 [accepted]
PHST- 2015/06/23 06:00 [entrez]
PHST- 2015/06/23 06:00 [pubmed]
PHST- 2016/06/28 06:00 [medline]
AID - 10.1002/elps.201500158 [doi]
PST - ppublish
SO  - Electrophoresis. 2015 Oct;36(19):2413-8. doi: 10.1002/elps.201500158. Epub 2015 
      Jul 29.