PMID- 26097626
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220310
IS  - 1937-6871 (Print)
IS  - 1937-688X (Electronic)
IS  - 1937-6871 (Linking)
VI  - 3
IP  - 11
DP  - 2010 Nov
TI  - Chondrogenic differentiation of stem cells in human umbilical cord stroma with 
      PGA and PLLA scaffolds.
PG  - 1041-1049
AB  - The stem cells in the umbilical cord stroma, or Wharton's jelly, are referred to 
      as human umbilical cord mesenchymal stromal cells (hUCMSCs) and have been shown 
      to differentiate along a chondrogenic lineage. The aim of this study was to 
      evaluate the chondrogenic differentiation of hUCMSCs in either polyglycolic acid 
      (PGA) or poly-L-lactic acid (PLLA) non-woven mesh scaffolds for cartilage tissue 
      engineering. PGA is widely known to degrade faster than PLLA, and over longer 
      time scales, and differences may be expected to emerge after extended culture 
      periods. Therefore, the focus of this study was to evaluate differences over a 
      shorter duration. After 21 days of culture in PLLA or PGA scaffolds, hUCMSC 
      constructs were analyzed for biochemical content, histology, and gene expression. 
      Overall, there were only minute differences between the two scaffold groups, with 
      similar gene expression and biosynthesis. The most notable difference was a 
      change in shape from cylindrical to spherical by the PGA, but not PLLA, scaffold 
      group. The overall similar behavior of the groups may suggest that in vivo 
      application of hUCMSC-seeded PLLA or PGA scaffolds, following a 21-day 
      pre-culture period, may yield similar constructs at the time of implantation. 
      However, differences may begin to become more apparent with in vivo performance 
      following implantation, or with in vitro performance over longer time periods.
FAU - Zhao, Liang
AU  - Zhao L
AD  - Department of Spinal & Orthopaedic Surgery, Southern Medical University, Nanfang 
      Hospital, Guangzhou, China ; Department of Chemical & Petroleum Engineering, 
      University of Kansas, Lawrence, KS, USA.
FAU - Detamore, Michael S
AU  - Detamore MS
AD  - Department of Spinal & Orthopaedic Surgery, Southern Medical University, Nanfang 
      Hospital, Guangzhou, China ; Department of Mechanical Engineering, University of 
      Kansas, Lawrence, KS, USA.
LA  - eng
GR  - R21 DE017673/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Biomed Sci Eng
JT  - Journal of biomedical science and engineering
JID - 101532098
PMC - PMC4474381
MID - NIHMS699716
OTO - NOTNLM
OT  - Cartilage Tissue Engineering
OT  - Scaffold
OT  - Stem Cells
OT  - Umbilical Cord
EDAT- 2010/11/01 00:00
MHDA- 2010/11/01 00:01
PMCR- 2015/06/19
CRDT- 2015/06/23 06:00
PHST- 2015/06/23 06:00 [entrez]
PHST- 2010/11/01 00:00 [pubmed]
PHST- 2010/11/01 00:01 [medline]
PHST- 2015/06/19 00:00 [pmc-release]
AID - 10.4236/jbise.2010.311135 [doi]
PST - ppublish
SO  - J Biomed Sci Eng. 2010 Nov;3(11):1041-1049. doi: 10.4236/jbise.2010.311135.