PMID- 26097834
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150622
LR  - 20200930
IS  - 2248-2997 (Print)
IS  - 2248-2997 (Electronic)
IS  - 2248-2997 (Linking)
VI  - 5
IP  - 2
DP  - 2015 Jun
TI  - HLA class II alleles in Romanian patients with chronic hepatitis C.
PG  - 44-9
LID - 10.11599/germs.2015.1070 [doi]
AB  - INTRODUCTION: The objective of the study was to determine the association of host 
      human leukocyte antigen (HLA) class II genotype DRB1 alleles with the response to 
      interferon therapy, viral loads and extent of liver fibrosis in a group of 
      Romanian patients diagnosed with chronic hepatitis C, with different clinical 
      outcomes. Class II HLA genes, particularly the HLA-DRB1 and DQB1 genes, have been 
      shown to have an important role in self-limiting or persistent viral infection, 
      in different genetic populations. In chronic hepatitis C both susceptible and 
      protective alleles have been described, influencing the development of 
      autoimmunity and progression to cirrhosis and hepatocellular carcinoma. METHODS: 
      The study included 54 patients diagnosed with chronic hepatitis C, registered and 
      monitored from January 2014 to January 2015 at the Clinical Hospital of 
      Infectious Diseases, Constanta, Romania. The selected patients were positive for 
      anti-HCV antibodies and HCV-RNA, with screening laboratory results indicating HCV 
      genotype 1b. The method used for the assignment of alleles at HLA-DRB1 and DQB1 
      loci was molecular genotyping, by the sequence specific oligonucleotide (SSO) 
      hybridization method, and when required, by the sequence specific primers method 
      (SSP). The presence of different alleles in patients has been analyzed for 
      statistical significance. RESULTS: The presence of HLA-DRB1*0301 had a high 
      frequency (14.8%) in null-responders (NR) while alleles DRB1*0701 (11.1%), 
      DRB1*11# (22.2%) and DRB1*0101 (16.7%) were prevalent in sustained virologic 
      responders (SVR). No significant correlation was found between the presence of 
      HLA-DRB1* alleles and viral loads or liver fibrosis with p values not 
      statistically significant after the Bonferroni correction. CONCLUSION: The 
      presented data suggest that in this group of Romanian patients, certain HLA 
      alleles influence the therapeutic response in HCV infection and genetic 
      predisposition may play a role in hepatitis C virus infection in those patients.
FAU - Gheorghe, Loredana
AU  - Gheorghe L
AD  - MD, Ovidius University of Constanta, Faculty of Medicine, Department of Cell 
      Biology and Medical Genetics.
FAU - Rugina, Sorin
AU  - Rugina S
AD  - MD, PhD, Ovidius University of Constanta, Faculty of Medicine, Department of 
      Infectious Diseases.
FAU - Dumitru, Irina Magdalena
AU  - Dumitru IM
AD  - MD, PhD, Ovidius University of Constanta, Faculty of Medicine, Department of 
      Infectious Diseases.
FAU - Franciuc, Irina
AU  - Franciuc I
AD  - MD, PhD, Ovidius University of Constanta, Faculty of Medicine, Department of Cell 
      Biology and Medical Genetics.
FAU - Martinescu, Alina
AU  - Martinescu A
AD  - MD, PhD, Ovidius University of Constanta, Faculty of Medicine, Department of Cell 
      Biology and Medical Genetics.
FAU - Balas, Iulia
AU  - Balas I
AD  - MD, Ovidius University of Constanta, Faculty of Medicine, Department of Cell 
      Biology and Medical Genetics.
LA  - eng
PT  - Journal Article
DEP - 20150602
PL  - Romania
TA  - Germs
JT  - Germs
JID - 101596099
PMC - PMC4472190
OTO - NOTNLM
OT  - Human leukocyte antigen
OT  - chronic hepatitis C
OT  - interferon response to HCV
COIS- Conflicts of interest: All authors - none to declare
EDAT- 2015/06/23 06:00
MHDA- 2015/06/23 06:01
PMCR- 2015/06/02
CRDT- 2015/06/23 06:00
PHST- 2015/03/30 00:00 [received]
PHST- 2015/05/14 00:00 [revised]
PHST- 2015/05/22 00:00 [accepted]
PHST- 2015/06/23 06:00 [entrez]
PHST- 2015/06/23 06:00 [pubmed]
PHST- 2015/06/23 06:01 [medline]
PHST- 2015/06/02 00:00 [pmc-release]
AID - germs.2015.1070 [pii]
AID - 10.11599/germs.2015.1070 [doi]
PST - epublish
SO  - Germs. 2015 Jun 2;5(2):44-9. doi: 10.11599/germs.2015.1070. eCollection 2015 Jun.