PMID- 26098687
OWN - NLM
STAT- MEDLINE
DCOM- 20160422
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 6
DP  - 2015
TI  - Litter Breakdown and Microbial Succession on Two Submerged Leaf Species in a 
      Small Forested Stream.
PG  - e0130801
LID - 10.1371/journal.pone.0130801 [doi]
LID - e0130801
AB  - Microbial succession during leaf breakdown was investigated in a small forested 
      stream in west-central Georgia, USA, using multiple culture-independent 
      techniques. Red maple (Acer rubrum) and water oak (Quercus nigra) leaf litter 
      were incubated in situ for 128 days, and litter breakdown was quantified by 
      ash-free dry mass (AFDM) method and microbial assemblage composition using 
      phospholipid fatty acid analysis (PLFA), ribosomal intergenic spacer analysis 
      (RISA), denaturing gradient gel electrophoresis (DGGE), and bar-coded 
      next-generation sequencing of 16S rRNA gene amplicons. Leaf breakdown was faster 
      for red maple than water oak. PLFA revealed a significant time effect on 
      microbial lipid profiles for both leaf species. Microbial assemblages on maple 
      contained a higher relative abundance of bacterial lipids than oak, and oak 
      microbial assemblages contained higher relative abundance of fungal lipids than 
      maple. RISA showed that incubation time was more important in structuring 
      bacterial assemblages than leaf physicochemistry. DGGE profiles revealed high 
      variability in bacterial assemblages over time, and sequencing of DGGE-resolved 
      amplicons indicated several taxa present on degrading litter. Next-generation 
      sequencing revealed temporal shifts in dominant taxa within the phylum 
      Proteobacteria, whereas gamma-Proteobacteria dominated pre-immersion and alpha- and 
      beta-Proteobacteria dominated after 1 month of instream incubation; the latter 
      groups contain taxa that are predicted to be capable of using organic material to 
      fuel further breakdown. Our results suggest that incubation time is more 
      important than leaf species physicochemistry in influencing leaf litter microbial 
      assemblage composition, and indicate the need for investigation into seasonal and 
      temporal dynamics of leaf litter microbial assemblage succession.
FAU - Newman, Molli M
AU  - Newman MM
AD  - Department of Biological Sciences, Auburn University, Auburn, Alabama, United 
      States of America.
FAU - Liles, Mark R
AU  - Liles MR
AD  - Department of Biological Sciences, Auburn University, Auburn, Alabama, United 
      States of America.
FAU - Feminella, Jack W
AU  - Feminella JW
AD  - Department of Biological Sciences, Auburn University, Auburn, Alabama, United 
      States of America.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150622
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Lipids)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Acer/*microbiology
MH  - Biodegradation, Environmental
MH  - Ecosystem
MH  - Forests
MH  - Fungi/genetics
MH  - Georgia
MH  - Lipids/genetics
MH  - Plant Leaves/*microbiology
MH  - Proteobacteria/genetics
MH  - Quercus/*microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rivers/*microbiology
PMC - PMC4476575
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/06/23 06:00
MHDA- 2016/04/23 06:00
PMCR- 2015/06/22
CRDT- 2015/06/23 06:00
PHST- 2014/12/12 00:00 [received]
PHST- 2015/05/25 00:00 [accepted]
PHST- 2015/06/23 06:00 [entrez]
PHST- 2015/06/23 06:00 [pubmed]
PHST- 2016/04/23 06:00 [medline]
PHST- 2015/06/22 00:00 [pmc-release]
AID - PONE-D-14-54995 [pii]
AID - 10.1371/journal.pone.0130801 [doi]
PST - epublish
SO  - PLoS One. 2015 Jun 22;10(6):e0130801. doi: 10.1371/journal.pone.0130801. 
      eCollection 2015.