PMID- 26100490
OWN - NLM
STAT- MEDLINE
DCOM- 20160805
LR  - 20220318
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 88
IP  - 1
DP  - 2016 Jan
TI  - Comparison of the conventional multiplex RT-PCR, real time RT-PCR and Luminex 
      xTAG(R) RVP fast assay for the detection of respiratory viruses.
PG  - 51-7
LID - 10.1002/jmv.24299 [doi]
AB  - Detection of respiratory viruses using polymerase chain reaction (PCR) is 
      sensitive, specific and cost effective, having huge potential for patient 
      management. In this study, the performance of an in-house developed conventional 
      multiplex RT-PCR (mRT-PCR), real time RT-PCR (rtRT-PCR) and Luminex xTAG((R)) RVP 
      fast assay (Luminex Diagnostics, Toronto, Canada) for the detection of 
      respiratory viruses was compared. A total 310 respiratory clinical specimens 
      predominantly from pediatric patients, referred for diagnosis of influenza 
      A/H1N1pdm09 from August 2009 to March 2011 were tested to determine performance 
      characteristic of the three methods. A total 193 (62.2%) samples were detected 
      positive for one or more viruses by mRT-PCR, 175 (56.4%) samples by real time 
      monoplex RT-PCR, and 138 (44.5%) samples by xTAG((R)) RVP fast assay. The overall 
      sensitivity of mRT-PCR was 96.9% (95% CI: 93.5, 98.8), rtRT-PCR 87.9% (95% CI: 
      82.5, 92.1) and xTAG((R)) RVP fast was 68.3% (95% CI: 61.4, 74.6). Rhinovirus was 
      detected most commonly followed by respiratory syncytial virus group B and 
      influenza A/H1N1pdm09. The monoplex real time RT-PCR and in-house developed 
      mRT-PCR are more sensitive, specific and cost effective than the xTAG((R)) RVP fast 
      assay.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Choudhary, Manohar L
AU  - Choudhary ML
AD  - National Institute of Virology, Human Influenza Group, Pune, India.
FAU - Anand, Siddharth P
AU  - Anand SP
AD  - National Institute of Virology, Human Influenza Group, Pune, India.
FAU - Tikhe, Shamal A
AU  - Tikhe SA
AD  - National Institute of Virology, Human Influenza Group, Pune, India.
FAU - Walimbe, Atul M
AU  - Walimbe AM
AD  - National Institute of Virology, Human Influenza Group, Pune, India.
FAU - Potdar, Varsha A
AU  - Potdar VA
AD  - National Institute of Virology, Human Influenza Group, Pune, India.
FAU - Chadha, Mandeep S
AU  - Chadha MS
AD  - National Institute of Virology, Human Influenza Group, Pune, India.
FAU - Mishra, Akhilesh C
AU  - Mishra AC
AD  - National Institute of Virology, Human Influenza Group, Pune, India.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
SB  - IM
MH  - Animals
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Infant
MH  - Male
MH  - Molecular Diagnostic Techniques/*methods
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Respiratory Tract Infections/diagnosis/*virology
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Virus Diseases/diagnosis/*virology
MH  - Viruses/*isolation & purification
PMC - PMC7166673
OTO - NOTNLM
OT  - RT-PCR
OT  - RVP assay
OT  - respiratory viruses
OT  - xTAG(R)
EDAT- 2015/06/24 06:00
MHDA- 2016/08/06 06:00
PMCR- 2020/04/19
CRDT- 2015/06/24 06:00
PHST- 2015/06/11 00:00 [accepted]
PHST- 2015/06/24 06:00 [entrez]
PHST- 2015/06/24 06:00 [pubmed]
PHST- 2016/08/06 06:00 [medline]
PHST- 2020/04/19 00:00 [pmc-release]
AID - JMV24299 [pii]
AID - 10.1002/jmv.24299 [doi]
PST - ppublish
SO  - J Med Virol. 2016 Jan;88(1):51-7. doi: 10.1002/jmv.24299.