PMID- 26102558 OWN - NLM STAT- MEDLINE DCOM- 20160427 LR - 20150730 IS - 1557-8542 (Electronic) IS - 1545-8547 (Linking) VI - 12 IP - 4 DP - 2015 Aug TI - Occurrence of Natural Hybrids Among Sympatric Karyomorphs in Hoplerythrinus unitaeniatus (Characiformes, Erythrinidae). PG - 281-7 LID - 10.1089/zeb.2015.1083 [doi] AB - In this study, 43 specimens of Hoplerythrinus unitaeniatus from the Sao Francisco River basin were chromosomally analyzed by conventional Giemsa staining, C-banding, silver nitrate impregnation, and fluorescence in situ hybridization (FISH) with probes of 5S and 18S rDNA. The diploid numbers found were 50 and 52 chromosomes, showing the existence of two well-defined biological entities in sympatry. Specimens with 51 chromosomes, which showed three distinct karyotypic forms, were also found and are characterized as natural hybrids due to the correspondence with the chromosomes of the specimens with 50 and 52 chromosomes. By FISH using 5S and 18S rDNA probes, it was possible to detect specific chromosomal markers for the specimens with 50 and 52 chromosomes, as well as the occurrence of common sites in both. The specimens with 51 chromosomes showed intermediate patterns for these markers, reinforcing the hypothesis that these are actual natural hybrids. A review and new classification for the karyomorphs of H. unitaeniatus have also been proposed. FAU - Martinez, Juliana de Fatima AU - Martinez Jde F AD - 1 Universidade Tecnologica Federal do Parana , Santa Helena, Parana, Brazil . FAU - Lui, Roberto Laridondo AU - Lui RL AD - 2 Centro de Ciencias Biologicas e da Saude, Universidade Estadual do Oeste do Parana , Cascavel, Parana, Brazil . FAU - Traldi, Josiane Baccarin AU - Traldi JB AD - 3 Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos , Sao Carlos, Sao Paulo, Brazil . FAU - Blanco, Daniel Rodrigues AU - Blanco DR AD - 1 Universidade Tecnologica Federal do Parana , Santa Helena, Parana, Brazil . FAU - Moreira-Filho, Orlando AU - Moreira-Filho O AD - 3 Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos , Sao Carlos, Sao Paulo, Brazil . LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150623 PL - United States TA - Zebrafish JT - Zebrafish JID - 101225070 RN - 0 (RNA, Ribosomal, 18S) RN - 0 (RNA, Ribosomal, 5S) SB - IM MH - Animals MH - Brazil MH - Characiformes/*genetics MH - Female MH - *Hybridization, Genetic MH - In Situ Hybridization, Fluorescence MH - Karyotype MH - Male MH - *Polymorphism, Genetic MH - RNA, Ribosomal, 18S/genetics MH - RNA, Ribosomal, 5S/genetics MH - Sympatry EDAT- 2015/06/24 06:00 MHDA- 2016/04/28 06:00 CRDT- 2015/06/24 06:00 PHST- 2015/06/24 06:00 [entrez] PHST- 2015/06/24 06:00 [pubmed] PHST- 2016/04/28 06:00 [medline] AID - 10.1089/zeb.2015.1083 [doi] PST - ppublish SO - Zebrafish. 2015 Aug;12(4):281-7. doi: 10.1089/zeb.2015.1083. Epub 2015 Jun 23.