PMID- 26104656
OWN - NLM
STAT- MEDLINE
DCOM- 20160421
LR  - 20181113
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 51
IP  - 1
DP  - 2015 Aug
TI  - Scylla serrata reovirus p35 protein expressed in Escherichia coli cells alters 
      membrane permeability.
PG  - 69-76
LID - 10.1007/s11262-015-1218-5 [doi]
AB  - To promote viral entry, replication, release, and spread to neighboring cells, 
      many cytolytic animal viruses encode proteins responsible for modification of 
      host cell membrane permeability and for formation of ion channels in host cell 
      membranes. Scylla serrata reovirus (SsRV) is a major pathogen that can severely 
      damage mud crab (S. serrata) aquaculture. Protein p35, which is encoded by 
      segment 10 of SsRV, contains two transmembrane domains. In this study, we found 
      that SsRV p35 can induce membrane permeability changes when expressed in 
      Escherichia coli. SsRV p35 expressed in bacterial cells existed as monomers under 
      reducing conditions but formed homodimers and homotrimers under non-reducing 
      conditions. These findings demonstrate that p35 may act as a viroporin; further 
      studies are needed to elucidate the detailed structure-function relationships of 
      this protein.
FAU - Zhang, Zhao
AU  - Zhang Z
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 
      8, South Qianhu Road, Ningbo, 315100, Zhejiang, People's Republic of China.
FAU - Yuan, Yangyang
AU  - Yuan Y
FAU - Fan, Dongyang
AU  - Fan D
FAU - Yang, Jifang
AU  - Yang J
FAU - Mao, Zhijuan
AU  - Mao Z
FAU - Yan, Yan
AU  - Yan Y
FAU - Chen, Jigang
AU  - Chen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150624
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Brachyura/virology
MH  - Cell Membrane/*drug effects/*physiology
MH  - Escherichia coli/genetics/*metabolism
MH  - Permeability/*drug effects
MH  - Protein Multimerization
MH  - Reoviridae/genetics/*physiology
MH  - Viral Proteins/genetics/*metabolism
EDAT- 2015/06/25 06:00
MHDA- 2016/04/22 06:00
CRDT- 2015/06/25 06:00
PHST- 2015/02/26 00:00 [received]
PHST- 2015/06/08 00:00 [accepted]
PHST- 2015/06/25 06:00 [entrez]
PHST- 2015/06/25 06:00 [pubmed]
PHST- 2016/04/22 06:00 [medline]
AID - 10.1007/s11262-015-1218-5 [doi]
PST - ppublish
SO  - Virus Genes. 2015 Aug;51(1):69-76. doi: 10.1007/s11262-015-1218-5. Epub 2015 Jun 
      24.