PMID- 26106283
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150624
LR  - 20200930
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 15
DP  - 2015
TI  - MicroRNA-24 inhibits growth, induces apoptosis, and reverses radioresistance in 
      laryngeal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis 
      protein.
PG  - 61
LID - 10.1186/s12935-015-0217-x [doi]
LID - 61
AB  - BACKGROUND: Increasing evidence indicates that dysregulation of microRNAs is 
      involved in tumor progression and development. The aim of this study was to 
      investigate the expression of microRNA-24 (miR-24) and its function in laryngeal 
      squamous cell carcinoma (LSCC). METHODS: Quantitative RT-PCR (qRT-PCR) was used 
      to detect miR-24 expression in LSCC cell lines and tissue samples. MTT, colony 
      formation, and flow cytometry was performed to analyze the effects of miR-24 
      expression on growth, apoptosis, and radiosensitivity of LSCC cells. 
      Dual-luciferase reporter assays were performed to examine regulation of putative 
      miR-24 targets. Expression of X-linked inhibitor of apoptosis protein (XIAP) mRNA 
      and protein, cleaved or total caspase-3, and cleaved or total PARP protein were 
      detected by qRT-PCR and western blotting assays, respectively. RESULTS: miR-24 
      expression levels in LSCC cell lines or tissue were significantly lower than in a 
      normal human keratinocyte cell line or adjacent normal tissues. Functional 
      analyses indicated that re-expression of miR-24 inhibits growth, reduces colony 
      formation, and enhances apoptosis in LSCC cells. In addition, miR-24 upregulation 
      increases LSCC sensitivity to irradiation by enhancing irradiation-induced 
      apoptosis, and luciferase activity indicated that miR-24 binds to the 
      3'-untranslated region (3'-UTR) of XIAP mRNA. Upregulation of miR-24 inhibits 
      XIAP protein expression in LSCC cells, and silencing of XIAP mimics the effects 
      of miR-24 upregulation on LSCC cells. In addition, XIAP mRNA expression 
      significantly increases in LSCC tissues and is inversely correlated with miR-24 
      expression. CONCLUSIONS: Our data suggest that miR-24 inhibits growth, increases 
      apoptosis, and enhances radiosensitivity in LSCC cells by targeting XIAP. 
      Therefore, miR-24 may be a potential molecular target for the treatment of human 
      LSCC.
FAU - Xu, Li
AU  - Xu L
AD  - Department of Otolaryngology-Head and Neck Surgery, Tangdu Hospital and 
      Laboratory for Laryngotracheal Reconstruction, Fourth Military Medical 
      University, Xi'an, Shaanxi 710038 PR China.
AD  - Department of Otolaryngology-Head and Neck Surgery, Nanjing General Hospital of 
      Nanjing Military Command, Nanjing, Jiangsu 210002 PR China.
FAU - Chen, Zhifeng
AU  - Chen Z
AD  - Department of Otolaryngology-Head and Neck Surgery, Lanzhou General Hospital of 
      Lanzhou Military Command, Lanzhou, Gansu 730050 PR China.
FAU - Xue, Fei
AU  - Xue F
AD  - Department of Otolaryngology-Head and Neck Surgery, Nanjing General Hospital of 
      Nanjing Military Command, Nanjing, Jiangsu 210002 PR China.
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Otolaryngology-Head and Neck Surgery, Nanjing General Hospital of 
      Nanjing Military Command, Nanjing, Jiangsu 210002 PR China.
FAU - Ma, Ruina
AU  - Ma R
AD  - Department of Otolaryngology-Head and Neck Surgery, Tangdu Hospital and 
      Laboratory for Laryngotracheal Reconstruction, Fourth Military Medical 
      University, Xi'an, Shaanxi 710038 PR China.
FAU - Cheng, Shiyin
AU  - Cheng S
AD  - Department of Otolaryngology-Head and Neck Surgery, Tangdu Hospital and 
      Laboratory for Laryngotracheal Reconstruction, Fourth Military Medical 
      University, Xi'an, Shaanxi 710038 PR China.
FAU - Cui, Pengcheng
AU  - Cui P
AD  - Department of Otolaryngology-Head and Neck Surgery, Tangdu Hospital and 
      Laboratory for Laryngotracheal Reconstruction, Fourth Military Medical 
      University, Xi'an, Shaanxi 710038 PR China.
LA  - eng
PT  - Journal Article
DEP - 20150617
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC4477309
OTO - NOTNLM
OT  - Apoptosis
OT  - Growth
OT  - Laryngeal squamous cell carcinoma
OT  - XIAP
OT  - miR-24
EDAT- 2015/06/25 06:00
MHDA- 2015/06/25 06:01
PMCR- 2015/06/17
CRDT- 2015/06/25 06:00
PHST- 2014/12/12 00:00 [received]
PHST- 2015/06/11 00:00 [accepted]
PHST- 2015/06/25 06:00 [entrez]
PHST- 2015/06/25 06:00 [pubmed]
PHST- 2015/06/25 06:01 [medline]
PHST- 2015/06/17 00:00 [pmc-release]
AID - 217 [pii]
AID - 10.1186/s12935-015-0217-x [doi]
PST - epublish
SO  - Cancer Cell Int. 2015 Jun 17;15:61. doi: 10.1186/s12935-015-0217-x. eCollection 
      2015.