PMID- 26107007
OWN - NLM
STAT- MEDLINE
DCOM- 20160826
LR  - 20220316
IS  - 1875-5941 (Electronic)
IS  - 0165-2176 (Linking)
VI  - 35
IP  - 4
DP  - 2015
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV) from the first 
      outbreak of India shows close relationship with the highly pathogenic variant of 
      China.
PG  - 186-93
LID - 10.1080/01652176.2015.1066043 [doi]
AB  - BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) is an 
      economically important viral disease of pigs worldwide. India has reported the 
      first outbreak of PRRS in the pig population of Mizoram state to the Office 
      International des Epizooties on the 26 June 2013. HYPOTHESIS/OBJECTIVES: The aim 
      of the present study was to determine the genotype and origin of porcine 
      reproductive and respiratory syndrome virus (PRRSV) from the first outbreak in 
      the pig population of Mizoram state, India, in 2013. ANIMALS AND METHODS: A total 
      of 880 affected pigs from the outbreak were clinically examined, 51 animals were 
      necropsied and tested by reverse transcription polymerase chain reaction (RT-PCR) 
      to detect PRRSV and 148 serum samples were tested to detect PRRSV-specific 
      antibodies. The full open reading frame 5 (ORF5) gene sequences from 12 and ORF7 
      gene sequences from three clinical cases were sequenced and analysed for genomic 
      characterization, respectively. RESULTS: The outbreak was confirmed by the 
      detection of PRRSV-specific antibodies in 109 out of 148 serum samples (74%) and 
      also by RT-PCR in 46 out of 51 necropsy samples (90%). Notably, ORF5 and ORF7 
      genes of Indian strain shares the same nucleotide positions i.e. 13,698-14,300 
      and 14,799-15,170, respectively, with the highly pathogenic (HP) strain of China 
      and were grouped together in a phylogenetic tree. CONCLUSIONS AND CLINICAL 
      IMPORTANCE: Sequence and phylogenetic analysis of ORF5 and ORF7 confirmed that 
      the Indian strain has a close link with the HP-PRRSV of China. The current study 
      forms an essential step for better understanding of the epidemiology as well as 
      the movement and spread of the disease in India.
FAU - Rajkhowa, T K
AU  - Rajkhowa TK
AD  - a Department of Veterinary Pathology, College of Veterinary Sciences & A.H. , 
      Central Agricultural University, Selesih , Aizawl , Mizoram , India.
FAU - Jagan Mohanarao, G
AU  - Jagan Mohanarao G
AD  - b Department of Veterinary Physiology and Biochemistry, College of Veterinary 
      Sciences & A.H. , Central Agricultural University, Selesih , Aizawl , Mizoram , 
      India.
FAU - Gogoi, A
AU  - Gogoi A
AD  - a Department of Veterinary Pathology, College of Veterinary Sciences & A.H. , 
      Central Agricultural University, Selesih , Aizawl , Mizoram , India.
FAU - Hauhnar, L
AU  - Hauhnar L
AD  - a Department of Veterinary Pathology, College of Veterinary Sciences & A.H. , 
      Central Agricultural University, Selesih , Aizawl , Mizoram , India.
FAU - Isaac, L
AU  - Isaac L
AD  - a Department of Veterinary Pathology, College of Veterinary Sciences & A.H. , 
      Central Agricultural University, Selesih , Aizawl , Mizoram , India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150716
PL  - England
TA  - Vet Q
JT  - The veterinary quarterly
JID - 7909485
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Disease Outbreaks/*veterinary
MH  - Female
MH  - *Genotype
MH  - India/epidemiology
MH  - Male
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Sequence Analysis, DNA/veterinary
MH  - Swine
MH  - Viral Proteins/*genetics/metabolism
OTO - NOTNLM
OT  - India
OT  - ORF5
OT  - ORF7
OT  - PRRS
OT  - phylogeny
OT  - pig
OT  - porcine
EDAT- 2015/06/25 06:00
MHDA- 2016/08/27 06:00
CRDT- 2015/06/25 06:00
PHST- 2015/06/25 06:00 [entrez]
PHST- 2015/06/25 06:00 [pubmed]
PHST- 2016/08/27 06:00 [medline]
AID - 10.1080/01652176.2015.1066043 [doi]
PST - ppublish
SO  - Vet Q. 2015;35(4):186-93. doi: 10.1080/01652176.2015.1066043. Epub 2015 Jul 16.