PMID- 26108712
OWN - NLM
STAT- MEDLINE
DCOM- 20161102
LR  - 20181113
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 41
IP  - 3
DP  - 2016 Mar
TI  - Changes in Hypoxia-Inducible Factor-1 (HIF-1) and Regulatory Prolyl Hydroxylase 
      (PHD) Enzymes Following Hypoxic-Ischemic Injury in the Neonatal Rat.
PG  - 515-22
LID - 10.1007/s11064-015-1641-y [doi]
AB  - Hypoxia leads to activation of many cellular adaptive processes which are 
      regulated by the transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 
      consists of HIF-1alpha and HIF-1ss subunits and levels of HIF-1alpha protein are regulated 
      by HIF prolyl-hydroxylase enzymes (PHD1, 2, 3). The aim of the current study was 
      to investigate the expression of HIF-1alpha and PHDs at various time points after 
      hypoxia-ischemia (HI), using a neonatal rat model of HI brain injury. 
      Sprague-Dawley rat pups (postnatal day 7) were anaesthetized and underwent right 
      carotid artery occlusion and were then exposed to 6 % oxygen for 2.5 h at 37  degrees C. 
      HI injured animals demonstrated a significant reduction in the size of the 
      ipsilateral hemisphere, compared to sham controls. Protein analysis using western 
      blotting and enzyme-linked immunosorbent assay showed that 24 h after HI, there 
      was a significant increase in PHD3 protein and an increase of HIF-1alpha compared to 
      controls. At the 72 h time point, there was a reduction in PHD3 protein, which 
      appeared to relate to cellular loss. There were no changes in PHD1 or PHD2 
      protein levels after HI when compared to age-matched controls. Further studies 
      are necessary to establish roles for the HIF-1 regulatory enzyme PHD3 in brain 
      injury processes.
FAU - Chu, Hannah X
AU  - Chu HX
AD  - Department of Pharmacology, Faculty of Medicine, Nursing and Health Services, 
      Monash University, Clayton, VIC, 3800, Australia.
FAU - Jones, Nicole M
AU  - Jones NM
AD  - Department of Pharmacology, School of Medical Sciences, UNSW Australia, Sydney, 
      NSW, 2052, Australia. n.jones@unsw.edu.au.
LA  - eng
PT  - Journal Article
DEP - 20150625
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Isoenzymes)
RN  - EC 1.14.11.- (Prolyl Hydroxylases)
RN  - EC 1.14.11.29 (EGLN3 protein, human)
RN  - EC 1.14.11.29 (Hypoxia-Inducible Factor-Proline Dioxygenases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cerebral Cortex/metabolism/pathology
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Hypoxia-Inducible Factor-Proline Dioxygenases/metabolism
MH  - Hypoxia-Ischemia, Brain/*metabolism/pathology
MH  - Isoenzymes/metabolism
MH  - Prolyl Hydroxylases/*metabolism
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Hypoxia-inducible factor-1
OT  - Hypoxia-ischemia
OT  - Neonatal brain injury
OT  - Prolyl hydroxylase
EDAT- 2015/06/26 06:00
MHDA- 2016/11/03 06:00
CRDT- 2015/06/26 06:00
PHST- 2015/03/20 00:00 [received]
PHST- 2015/06/15 00:00 [accepted]
PHST- 2015/06/12 00:00 [revised]
PHST- 2015/06/26 06:00 [entrez]
PHST- 2015/06/26 06:00 [pubmed]
PHST- 2016/11/03 06:00 [medline]
AID - 10.1007/s11064-015-1641-y [pii]
AID - 10.1007/s11064-015-1641-y [doi]
PST - ppublish
SO  - Neurochem Res. 2016 Mar;41(3):515-22. doi: 10.1007/s11064-015-1641-y. Epub 2015 
      Jun 25.