PMID- 26110477
OWN - NLM
STAT- MEDLINE
DCOM- 20170130
LR  - 20170130
IS  - 1848-6312 (Electronic)
IS  - 0004-1254 (Linking)
VI  - 66
IP  - 2
DP  - 2015 Jun
TI  - Genotoxic effects of diazinon on human peripheral blood lymphocytes.
PG  - 153-8
LID - /j/aiht.2015.66.issue-2/aiht-2015-66-2584/aiht-2015-66-2584.xml [pii]
LID - 10.1515/aiht-2015-66-2584 [doi]
AB  - The aim of this study was to evaluate the genetic damage in human peripheral 
      blood lymphocytes following 24 and 48- hour exposure to a commercial diazinon 
      formulation Basudin 60EM(R) at concentrations between 0.01 and 40 mug mL-1. For this 
      purpose we used the micronucleus (MN), fluorescence in situ hybridization (FISH), 
      and alkaline single cell gel electrophoresis (comet) assay. Diazinon 
      significantly increased the frequency of micronucleated cells compared to 
      control. Forty-eight-hour exposure increased this frequency even at lower 
      concentrations (0.01-10 mug mL-1). The FISH results revealed aneugenic effects at 
      10 mug mL-1. The comet assay also confirmed DNA damage at concentrations between 
      10 and 40 mug mL-1. Our findings have confirmed the genotoxic potential of 
      diazinon and its cytotoxic effect on human lymphocytes. The increased DNA damage 
      in our study raises concern about the current assessment of the health risk posed 
      by this pesticide and calls for a high level of caution in agricultural and 
      household use.
FAU - Muranli, Fulya Dilek Gokalp
AU  - Muranli FD
FAU - Kanev, Martin
AU  - Kanev M
FAU - Ozdemir, Kezban
AU  - Ozdemir K
LA  - eng
PT  - Journal Article
PL  - Croatia
TA  - Arh Hig Rada Toksikol
JT  - Arhiv za higijenu rada i toksikologiju
JID - 0373100
RN  - 0 (Insecticides)
RN  - 0 (Mutagens)
RN  - YUS1M1Q929 (Diazinon)
SB  - IM
MH  - Agriculture
MH  - DNA Damage/*drug effects
MH  - Diazinon/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Household Products
MH  - Humans
MH  - Insecticides/*toxicity
MH  - Lymphocytes/*drug effects
MH  - Mutagens/*toxicity
MH  - Occupational Exposure/*adverse effects
EDAT- 2015/06/26 06:00
MHDA- 2017/01/31 06:00
CRDT- 2015/06/26 06:00
PHST- 2014/10/01 00:00 [received]
PHST- 2015/05/01 00:00 [accepted]
PHST- 2015/06/26 06:00 [entrez]
PHST- 2015/06/26 06:00 [pubmed]
PHST- 2017/01/31 06:00 [medline]
AID - /j/aiht.2015.66.issue-2/aiht-2015-66-2584/aiht-2015-66-2584.xml [pii]
AID - 10.1515/aiht-2015-66-2584 [doi]
PST - ppublish
SO  - Arh Hig Rada Toksikol. 2015 Jun;66(2):153-8. doi: 10.1515/aiht-2015-66-2584.