PMID- 26112242
OWN - NLM
STAT- MEDLINE
DCOM- 20160615
LR  - 20171116
IS  - 1522-1601 (Electronic)
IS  - 0161-7567 (Linking)
VI  - 119
IP  - 6
DP  - 2015 Sep 15
TI  - Effects of sleeping with reduced carbohydrate availability on acute training 
      responses.
PG  - 643-55
LID - 10.1152/japplphysiol.00857.2014 [doi]
AB  - We determined the effects of "periodized nutrition" on skeletal muscle and whole 
      body responses to a bout of prolonged exercise the following morning. Seven 
      cyclists completed two trials receiving isoenergetic diets differing in the 
      timing of ingestion: they consumed either 8 g/kg body mass (BM) of carbohydrate 
      (CHO) before undertaking an evening session of high-intensity training (HIT) and 
      slept without eating (FASTED), or consumed 4 g/kg BM of CHO before HIT, then 4 
      g/kg BM of CHO before sleeping (FED). The next morning subjects completed 2 h of 
      cycling (120SS) while overnight fasted. Muscle biopsies were taken on day 1 (D1) 
      before and 2 h after HIT and on day 2 (D2) pre-, post-, and 4 h after 120SS. 
      Muscle [glycogen] was higher in FED at all times post-HIT (P < 0.001). The 
      cycling bouts increased PGC1alpha mRNA and PDK4 mRNA (P < 0.01) in both trials, with 
      PDK4 mRNA being elevated to a greater extent in FASTED (P < 0.05). Resting 
      phosphorylation of AMPK(Thr172), p38MAPK(Thr180/Tyr182), and p-ACC(Ser79) (D2) 
      was greater in FASTED (P < 0.05). Fat oxidation during 120SS was higher in FASTED 
      (P = 0.01), coinciding with increases in ACC(Ser79) and CPT1 as well as mRNA 
      expression of CD36 and FABP3 (P < 0.05). Methylation on the gene promoter for 
      COX4I1 and FABP3 increased 4 h after 120SS in both trials, whereas methylation of 
      the PPARdelta promoter increased only in FASTED. We provide evidence for shifts in 
      DNA methylation that correspond with inverse changes in transcription for 
      metabolically adaptive genes, although delaying postexercise feeding failed to 
      augment markers of mitochondrial biogenesis.
CI  - Copyright (c) 2015 the American Physiological Society.
FAU - Lane, Stephen C
AU  - Lane SC
AD  - Exercise and Nutrition Research Group, School of Medical Sciences, RMIT 
      University, Bundoora, Australia;
FAU - Camera, Donny M
AU  - Camera DM
AD  - Centre for Exercise and Nutrition, Mary MacKillop Health Research Institute, 
      Australian Catholic University, Melbourne, Australia;
FAU - Lassiter, David Gray
AU  - Lassiter DG
AD  - Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden;
FAU - Areta, Jose L
AU  - Areta JL
AD  - Exercise and Nutrition Research Group, School of Medical Sciences, RMIT 
      University, Bundoora, Australia;
FAU - Bird, Stephen R
AU  - Bird SR
AD  - Exercise and Nutrition Research Group, School of Medical Sciences, RMIT 
      University, Bundoora, Australia;
FAU - Yeo, Wee Kian
AU  - Yeo WK
AD  - National Sports Institute of Malaysia, Kuala Lumpur, Malaysia;
FAU - Jeacocke, Nikki A
AU  - Jeacocke NA
AD  - Sports Nutrition, Australian Institute of Sport, Belconnen, Australia;
FAU - Krook, Anna
AU  - Krook A
AD  - Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; and.
FAU - Zierath, Juleen R
AU  - Zierath JR
AD  - Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden; 
      Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; and.
FAU - Burke, Louise M
AU  - Burke LM
AD  - Sports Nutrition, Australian Institute of Sport, Belconnen, Australia;
FAU - Hawley, John A
AU  - Hawley JA
AD  - Centre for Exercise and Nutrition, Mary MacKillop Health Research Institute, 
      Australian Catholic University, Melbourne, Australia; Institute for Sport and 
      Exercise Sciences, Liverpool John Moores University, Liverpool, United Kingdom 
      john.hawley@acu.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150625
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (RNA, Messenger)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (pyruvate dehydrogenase kinase 4)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Adaptation, Physiological/physiology
MH  - Adult
MH  - DNA Methylation/physiology
MH  - Dietary Carbohydrates/*metabolism
MH  - Exercise/*physiology
MH  - Glycogen/metabolism
MH  - Humans
MH  - Male
MH  - Muscle, Skeletal/metabolism/physiology
MH  - Phosphorylation/physiology
MH  - Physical Endurance/*physiology
MH  - Protein Kinases/metabolism
MH  - RNA, Messenger/metabolism
MH  - Sleep/*physiology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - HIT
OT  - cycling
OT  - muscle glycogen
OT  - sleep-low
OT  - train low
EDAT- 2015/06/27 06:00
MHDA- 2016/06/16 06:00
CRDT- 2015/06/27 06:00
PHST- 2014/09/24 00:00 [received]
PHST- 2015/06/22 00:00 [accepted]
PHST- 2015/06/27 06:00 [entrez]
PHST- 2015/06/27 06:00 [pubmed]
PHST- 2016/06/16 06:00 [medline]
AID - japplphysiol.00857.2014 [pii]
AID - 10.1152/japplphysiol.00857.2014 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2015 Sep 15;119(6):643-55. doi: 
      10.1152/japplphysiol.00857.2014. Epub 2015 Jun 25.