PMID- 26113285
OWN - NLM
STAT- MEDLINE
DCOM- 20160412
LR  - 20220408
IS  - 1872-8227 (Electronic)
IS  - 0168-8227 (Linking)
VI  - 109
IP  - 3
DP  - 2015 Sep
TI  - Impact of the hypoxia inducible factor-1alpha (HIF-1alpha) pro582ser polymorphism and its 
      gene expression on diabetic foot ulcers.
PG  - 533-40
LID - S0168-8227(15)00244-2 [pii]
LID - 10.1016/j.diabres.2015.05.014 [doi]
AB  - AIM: Adaptation to low oxygen tension (hypoxia) in cells and tissues leads to the 
      transcriptional induction of series of genes and the primary factor mediating 
      this response is the hypoxia-inducible factor-1alpha. This study was designed in 
      order to examine the HIF-1alpha gene polymorphism, p582s (rs11549465) in Exon-12 of 
      HIF-1alpha gene in diabetic subjects with and without foot ulcers (DFU) and to find 
      its expression under these pathological conditions. METHODS: A total of 224 
      subjects from our tertiary care centre were included, which consists of healthy 
      controls (N=66), type 2 diabetes mellitus (T2DM) (N=79) and T2DM with foot ulcers 
      (DFU) (N=79). Allelic and genotypic comparisons between the different groups were 
      evaluated by PCR-RFLP. The gene expression studies on selected samples (N=15 of 
      each group) were done by Semi-quantitative real time PCR. RESULTS AND 
      DISCUSSIONS: Genotype analysis showed a significant increase in presence of 'T' 
      allele in T2DM & DFU when compared to that of control subjects. Allele wise 
      analysis showed a higher frequency of 'T' allele in the T2DM (62.03%) when 
      compared to that of control subjects (53.79%). Interestingly, semi-quantitative 
      RT-PCR results showed decreased expression of HIF-1alpha gene on DFU when compared to 
      that of T2DM and control subjects. CONCLUSION: Our findings predict that there is 
      an association of HIF-1alpha gene polymorphism on foot ulcer patients when compare to 
      that of healthy controls. Semi-quantitative real time studies showed decreased 
      HIF-1alpha gene expression on foot ulcer patients suggesting its possible role on the 
      pathogenesis.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Pichu, Sivakamasundari
AU  - Pichu S
AD  - Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, 
      Royapuram, Chennai 600013, Tamil Nadu, India. Electronic address: 
      au_sudha@yahoo.com.
FAU - Sathiyamoorthy, Jayalalitha
AU  - Sathiyamoorthy J
AD  - Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, 
      Royapuram, Chennai 600013, Tamil Nadu, India.
FAU - Krishnamoorthy, Ezhilarasi
AU  - Krishnamoorthy E
AD  - Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, 
      Royapuram, Chennai 600013, Tamil Nadu, India.
FAU - Umapathy, Dhamodharan
AU  - Umapathy D
AD  - Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, 
      Royapuram, Chennai 600013, Tamil Nadu, India.
FAU - Viswanathan, Vijay
AU  - Viswanathan V
AD  - Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, 
      Royapuram, Chennai 600013, Tamil Nadu, India. Electronic address: 
      dr_vijay@mvdiabetes.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150522
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 452VLY9402 (Serine)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Substitution/genetics
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/complications/epidemiology/genetics
MH  - Diabetic Foot/epidemiology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - Proline/genetics
MH  - Serine/genetics
OTO - NOTNLM
OT  - DFU
OT  - HIF-1alpha
OT  - Hypoxia
OT  - SNP
OT  - T2DM
EDAT- 2015/06/27 06:00
MHDA- 2016/04/14 06:00
CRDT- 2015/06/27 06:00
PHST- 2014/08/08 00:00 [received]
PHST- 2015/04/08 00:00 [revised]
PHST- 2015/05/03 00:00 [accepted]
PHST- 2015/06/27 06:00 [entrez]
PHST- 2015/06/27 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
AID - S0168-8227(15)00244-2 [pii]
AID - 10.1016/j.diabres.2015.05.014 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2015 Sep;109(3):533-40. doi: 
      10.1016/j.diabres.2015.05.014. Epub 2015 May 22.