PMID- 26116380
OWN - NLM
STAT- MEDLINE
DCOM- 20160401
LR  - 20181113
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 11
DP  - 2015 Jun 27
TI  - mRNA expression of genes involved in inflammation and haemostasis in equine 
      fibroblast-like synoviocytes following exposure to lipopolysaccharide, fibrinogen 
      and thrombin.
PG  - 141
LID - 10.1186/s12917-015-0448-z [doi]
LID - 141
AB  - BACKGROUND: Studies in humans have shown that haemostatic and inflammatory 
      pathways both play important roles in the pathogenesis of joint disease. The aim 
      of this study was to assess mRNA expression of haemostatic and inflammatory 
      factors in cultured equine fibroblast-like synoviocytes exposed to 
      lipopolysaccharide (LPS), fibrinogen and thrombin. Synovial membranes were 
      collected from metacarpo-phalangeal joints of 6 skeletally mature horses 
      euthanized for non-orthopaedic reasons. Passage 4 fibroblast-like synoviocytes 
      were left non-treated or treated with either 0.1 mug/ml LPS, 5 mg/ml fibrinogen or 
      5 U/ml thrombin and harvested at time points 0, 6, 24 and 48 h. mRNA expression 
      of serum amyloid A (SAA), interleukin-6 (IL-6), monocyte chemotactic protein 1 
      (MCP-1), tissue factor (TF), plasminogen activator inhibitor 1 (PAI-1), urokinase 
      plasminogen activator (uPA), vascular endothelial growth factor (VEGF) and 
      protease activator receptor 1 (PAR-1) was assessed using quantitative real time 
      reverse transcriptase PCR. RESULTS: LPS caused a significant increase in mRNA 
      expression of SAA, IL-6, MCP-1 and uPA, and a decrease in TF, PAI-1 and PAR-1 
      when compared to non-treated cells. Treatment with thrombin resulted in increased 
      mRNA expression of SAA, IL-6, MCP-1 and PAI-1, and a decreased PAR-1 expression 
      compared to non-treated cells. The fibrinogen-treated synoviocytes showed 
      significantly increased mRNA expression of IL-6, MCP-1, TF and PAI-1, and 
      decreased PAR-1 expression compared to non-treated cells. CONCLUSION: LPS, 
      fibrinogen and thrombin induced an increased gene expression of inflammatory 
      markers in isolated equine fibroblast-like synoviocytes. LPS caused changes in 
      gene expression promoting increased fibrinolysis, while fibrinogen and thrombin 
      changed the gene expression resulting potentially in reduced fibrinolysis. 
      Overall, it appeared that both inflammatory and haemostatic stimuli affected 
      expression of genes involved in inflammatory and haemostatic pathways, supporting 
      their importance in equine joint diseases.
FAU - Andreassen, Stine Mandrup
AU  - Andreassen SM
AD  - Department of Large Animal Sciences, Medicine and Surgery group, University of 
      Copenhagen, Hojbakkegard alle 5, DK-2630, Tastrup, Denmark. 
      andreassenstine@hotmail.com.
FAU - Berg, Lise C
AU  - Berg LC
AD  - Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, 
      Dyrlaegevej 16, DK-1870, Frederiksberg C, Denmark. lcb@sund.ku.dk.
FAU - Nielsen, Soren Saxmose
AU  - Nielsen SS
AD  - Department of Large Animal Sciences, University of Copenhagen, Gronnegardsvej 8, 
      DK-1870, Frederiksberg C, Denmark. saxmose@sund.ku.dk.
FAU - Kristensen, Annemarie T
AU  - Kristensen AT
AD  - Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, 
      Dyrlaegevej 16, DK-1870, Frederiksberg C, Denmark. atk@sund.ku.dk.
FAU - Jacobsen, Stine
AU  - Jacobsen S
AD  - Department of Large Animal Sciences, Medicine and Surgery group, University of 
      Copenhagen, Hojbakkegard alle 5, DK-2630, Tastrup, Denmark. stj@sund.ku.dk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150627
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 9001-32-5 (Fibrinogen)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Fibrinogen/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Hemostasis/drug effects/physiology
MH  - *Horses
MH  - Inflammation/metabolism
MH  - Lipopolysaccharides/*toxicity
MH  - RNA, Messenger/genetics/*metabolism
MH  - Synovial Membrane/*cytology
MH  - Thrombin/*pharmacology
PMC - PMC4483216
EDAT- 2015/06/28 06:00
MHDA- 2016/04/02 06:00
PMCR- 2015/06/27
CRDT- 2015/06/28 06:00
PHST- 2014/09/23 00:00 [received]
PHST- 2015/06/01 00:00 [accepted]
PHST- 2015/06/28 06:00 [entrez]
PHST- 2015/06/28 06:00 [pubmed]
PHST- 2016/04/02 06:00 [medline]
PHST- 2015/06/27 00:00 [pmc-release]
AID - 10.1186/s12917-015-0448-z [pii]
AID - 448 [pii]
AID - 10.1186/s12917-015-0448-z [doi]
PST - epublish
SO  - BMC Vet Res. 2015 Jun 27;11:141. doi: 10.1186/s12917-015-0448-z.