PMID- 26116680
OWN - NLM
STAT- MEDLINE
DCOM- 20160208
LR  - 20181113
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 81
IP  - 17
DP  - 2015 Sep 1
TI  - Composition of Bacterial Communities Associated with Aurelia aurita Changes with 
      Compartment, Life Stage, and Population.
PG  - 6038-52
LID - 10.1128/AEM.01601-15 [doi]
AB  - The scyphozoan Aurelia aurita is recognized as a key player in marine ecosystems 
      and a driver of ecosystem change. It is thus intensely studied to address 
      ecological questions, although its associations with microorganisms remain so far 
      undescribed. In the present study, the microbiota associated with A. aurita was 
      visualized with fluorescence in situ hybridization (FISH) analysis, and community 
      structure was analyzed with respect to different life stages, compartments, and 
      populations of A. aurita by 16S rRNA gene amplicon sequencing. We demonstrate 
      that the composition of the A. aurita microbiota is generally highly distinct 
      from the composition of communities present in ambient water. Comparison of 
      microbial communities from different developmental stages reveals evidence for 
      life stage-specific community patterns. Significant restructuring of the 
      microbiota during strobilation from benthic polyp to planktonic life stages is 
      present, arguing for a restructuring during the course of metamorphosis. 
      Furthermore, the microbiota present in different compartments of the adult medusa 
      (exumbrella mucus and gastric cavity) display significant differences, indicating 
      body part-specific colonization. A novel Mycoplasma strain was identified in both 
      compartment-specific microbiota and is most likely present inside the epithelium 
      as indicated by FISH analysis of polyps, indicating potential endosymbiosis. 
      Finally, comparison of polyps of different populations kept under the same 
      controlled laboratory conditions in the same ambient water showed 
      population-specific community patterns, most likely due the genetic background of 
      the host. In conclusion, the presented data indicate that the associated 
      microbiota of A. aurita may play important functional roles, e.g., during the 
      life cycle.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Weiland-Brauer, Nancy
AU  - Weiland-Brauer N
AD  - Institute for General Microbiology, Christian Albrecht University Kiel, Kiel, 
      Germany.
FAU - Neulinger, Sven C
AU  - Neulinger SC
AD  - Institute for General Microbiology, Christian Albrecht University Kiel, Kiel, 
      Germany.
FAU - Pinnow, Nicole
AU  - Pinnow N
AD  - Institute for General Microbiology, Christian Albrecht University Kiel, Kiel, 
      Germany.
FAU - Kunzel, Sven
AU  - Kunzel S
AD  - Max Planck Institute for Evolutionary Biology, Plon, Germany.
FAU - Baines, John F
AU  - Baines JF
AD  - Max Planck Institute for Evolutionary Biology, Plon, Germany Institute for 
      Experimental Medicine, Christian Albrecht University Kiel, Kiel, Germany.
FAU - Schmitz, Ruth A
AU  - Schmitz RA
AD  - Institute for General Microbiology, Christian Albrecht University Kiel, Kiel, 
      Germany rschmitz@ifam.uni-kiel.de.
LA  - eng
SI  - GENBANK/KP299289
SI  - SRA/SRP049658
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150626
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - DNA, Bacterial/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Microbiota
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Scyphozoa/*growth & development/*microbiology
PMC - PMC4551246
EDAT- 2015/06/28 06:00
MHDA- 2016/02/09 06:00
PMCR- 2016/03/01
CRDT- 2015/06/28 06:00
PHST- 2015/05/15 00:00 [received]
PHST- 2015/06/19 00:00 [accepted]
PHST- 2015/06/28 06:00 [entrez]
PHST- 2015/06/28 06:00 [pubmed]
PHST- 2016/02/09 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - AEM.01601-15 [pii]
AID - 01601-15 [pii]
AID - 10.1128/AEM.01601-15 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2015 Sep 1;81(17):6038-52. doi: 10.1128/AEM.01601-15. 
      Epub 2015 Jun 26.