PMID- 26116899
OWN - NLM
STAT- MEDLINE
DCOM- 20160525
LR  - 20150813
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 208
DP  - 2015 Oct 2
TI  - Genome-wide transcriptional profiling reveals that HIV-1 Vpr differentially 
      regulates interferon-stimulated genes in human monocyte-derived dendritic cells.
PG  - 156-63
LID - S0168-1702(15)00282-8 [pii]
LID - 10.1016/j.virusres.2015.06.017 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that directly 
      link the innate and adaptive immune responses. HIV-1 infection of DCs leads to a 
      diverse array of changes in gene expression and play a major role in 
      dissemination of the virus into T-cells. Although HIV-1 Vpr is a pleiotropic 
      protein involved in HIV-1 replication and pathogenesis, its exact role in APCs 
      such as DCs remains elusive. In this study, utilizing a microarray-based systemic 
      biology approach, we found that HIV-1 Vpr differentially regulates (fold change 
      >2.0) more than 200 genes, primarily those involved in the immune response and 
      innate immune response including type I interferon signaling pathway. The 
      differential expression profiles of select genes involved in innate immune 
      responses (interferon-stimulated genes [ISGs]), including MX1, MX2, ISG15, ISG20, 
      IFIT1, IFIT2, IFIT3, IFI27, IFI44L, and TNFSF10, were validated by real-time 
      quantitative PCR; the results were consistent with the microarray data. Taken 
      together, our findings are the first to demonstrate that HIV-1 Vpr induces ISGs 
      and activates the type I IFN signaling pathway in human DCs, and provide insights 
      into the role of Vpr in HIV-1 pathogenesis.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Zahoor, Muhammad Atif
AU  - Zahoor MA
AD  - Viral Infectious Diseases Unit, RIKEN, Wako, Saitama 351-0198, Japan; 
      International Research Fellow of the Japan Society for the Promotion of Science, 
      Tokyo, Japan.
FAU - Xue, Guangai
AU  - Xue G
AD  - Viral Infectious Diseases Unit, RIKEN, Wako, Saitama 351-0198, Japan; 
      International Research Fellow of the Japan Society for the Promotion of Science, 
      Tokyo, Japan; Japanese Foundation of AIDS Prevention, Tokyo, Japan.
FAU - Sato, Hirotaka
AU  - Sato H
AD  - Viral Infectious Diseases Unit, RIKEN, Wako, Saitama 351-0198, Japan.
FAU - Aida, Yoko
AU  - Aida Y
AD  - Viral Infectious Diseases Unit, RIKEN, Wako, Saitama 351-0198, Japan. Electronic 
      address: aida@riken.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150625
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (vpr Gene Products, Human Immunodeficiency Virus)
RN  - 0 (vpr protein, Human immunodeficiency virus 1)
SB  - IM
MH  - Dendritic Cells/cytology/*immunology
MH  - Genome, Human
MH  - HIV Infections/*genetics/immunology/virology
MH  - HIV-1/genetics/*metabolism
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Immunity, Innate
MH  - Interferon Regulatory Factors/*genetics/immunology
MH  - Monocytes/cytology/*immunology
MH  - vpr Gene Products, Human Immunodeficiency Virus/genetics/*metabolism
OTO - NOTNLM
OT  - HIV-1 Vpr
OT  - Immune response
OT  - Interferon stimulation genes (ISGs)
OT  - Microarray
OT  - Monocyte-derived dendritic cells
OT  - Recombinant adenoviral vector
OT  - Type I interferon
EDAT- 2015/06/28 06:00
MHDA- 2016/05/26 06:00
CRDT- 2015/06/28 06:00
PHST- 2015/04/23 00:00 [received]
PHST- 2015/06/17 00:00 [revised]
PHST- 2015/06/17 00:00 [accepted]
PHST- 2015/06/28 06:00 [entrez]
PHST- 2015/06/28 06:00 [pubmed]
PHST- 2016/05/26 06:00 [medline]
AID - S0168-1702(15)00282-8 [pii]
AID - 10.1016/j.virusres.2015.06.017 [doi]
PST - ppublish
SO  - Virus Res. 2015 Oct 2;208:156-63. doi: 10.1016/j.virusres.2015.06.017. Epub 2015 
      Jun 25.