PMID- 26117152
OWN - NLM
STAT- MEDLINE
DCOM- 20160418
LR  - 20210119
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 33
IP  - 32
DP  - 2015 Jul 31
TI  - Deoxynivalenol (DON) naturally contaminated feed impairs the immune response 
      induced by porcine reproductive and respiratory syndrome virus (PRRSV) live 
      attenuated vaccine.
PG  - 3881-6
LID - S0264-410X(15)00870-1 [pii]
LID - 10.1016/j.vaccine.2015.06.069 [doi]
AB  - Cereal commodities are frequently contaminated with mycotoxins produced by the 
      secondary metabolism of fungal infection. Among these contaminants, 
      deoxynivalenol (DON), also known as vomitoxin, is the most prevalent type B 
      trichothecene mycotoxin worldwide. Pigs are very sensitive to the toxic effects 
      of DON and are frequently exposed to naturally contaminated feed. Recently, DON 
      naturally contaminated feed has been shown to decrease porcine reproductive and 
      respiratory syndrome virus (PRRSV) specific antibody responses following 
      experimental infection. The objective of this study was to determine the impact 
      of DON naturally contaminated feed on the immune response generated following 
      vaccination with PRRSV live attenuated vaccine. Eighteen pigs were randomly 
      divided into three experimental groups of 6 animals based on DON content of the 
      diets (0, 2.5 and 3.5mg DON/kg). They were fed these rations one week prior to 
      the vaccination and for all the duration of the immune response evaluation. All 
      pigs were vaccinated intra-muscularly with one dose of Ingelvac((R)) PRRSV modified 
      live vaccine (MLV). Blood samples were collected at day -1, 6, 13, 20, 27 and 35 
      post vaccination (pv) and tested for PRRSV RNA by RT-qPCR and for virus specific 
      antibodies by ELISA. Results showed that ingestion of DON-contaminated diets 
      significantly decreased PRRSV viremia. All pigs fed control diet were viremic 
      while only 1 (17%) and 3 (50%) out of 6 pigs were viremic in the groups receiving 
      3.5 and 2.5mg of DON/kg, respectively. Subsequently, all pigs fed control diet 
      developed PRRSV specific antibodies while only viremic pigs that were fed 
      contaminated diets have developed PRRSV specific antibodies. These results 
      suggest that feeding pigs with DON-contaminated diet could inhibit vaccination 
      efficiency of PRRSV MLV by severely impairing viral replication.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Savard, Christian
AU  - Savard C
AD  - Groupe de recherche sur les maladies infectieuses du porc (GREMIP); Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Faculte de medecine 
      veterinaire, Universite de Montreal, Saint-Hyacinthe, QC, Canada.
FAU - Gagnon, Carl A
AU  - Gagnon CA
AD  - Groupe de recherche sur les maladies infectieuses du porc (GREMIP); Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Faculte de medecine 
      veterinaire, Universite de Montreal, Saint-Hyacinthe, QC, Canada.
FAU - Chorfi, Younes
AU  - Chorfi Y
AD  - Departement de biomedecine veterinaire, Faculte de medecine veterinaire, 
      Universite de Montreal, Saint-Hyacinthe, QC, Canada. Electronic address: 
      younes.chorfi@umontreal.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150625
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antiviral Agents)
RN  - 0 (RNA, Viral)
RN  - 0 (Trichothecenes)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Antiviral Agents/*administration & dosage
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Food Contamination
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - RNA, Viral/blood/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Swine
MH  - Trichothecenes/*administration & dosage
MH  - Vaccines, Attenuated/administration & dosage/immunology
MH  - Viral Vaccines/*administration & dosage/*immunology
PMC - PMC7127064
OTO - NOTNLM
OT  - DON mycotoxin
OT  - PRRSV
OT  - Pig
OT  - Vaccination
EDAT- 2015/06/29 06:00
MHDA- 2016/04/19 06:00
PMCR- 2015/06/25
CRDT- 2015/06/29 06:00
PHST- 2015/02/23 00:00 [received]
PHST- 2015/06/10 00:00 [revised]
PHST- 2015/06/15 00:00 [accepted]
PHST- 2015/06/29 06:00 [entrez]
PHST- 2015/06/29 06:00 [pubmed]
PHST- 2016/04/19 06:00 [medline]
PHST- 2015/06/25 00:00 [pmc-release]
AID - S0264-410X(15)00870-1 [pii]
AID - 10.1016/j.vaccine.2015.06.069 [doi]
PST - ppublish
SO  - Vaccine. 2015 Jul 31;33(32):3881-6. doi: 10.1016/j.vaccine.2015.06.069. Epub 2015 
      Jun 25.