PMID- 26117943
OWN - NLM
STAT- MEDLINE
DCOM- 20150925
LR  - 20190318
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 21
IP  - 5
DP  - 2015 May
TI  - [Morinda officinalis extract repairs cytoxan-impaired spermatogenesis of male 
      rats].
PG  - 436-42
AB  - OBJECTIVE: To study the effect of Morinda officinalis (MO) extract on cytoxan 
      (CTX) -impaired spermatogenesis of adult male SD rats. METHODS: We randomly 
      divided 56 adult male SD rats into seven groups of equal number: blank control, 
      CTX model, CTX + NS, CTX + 10 g/kg MO, CTX + 20 g/kg MO, CTX + 30 g/kg MO, and 
      CTX + 40 g/kg MO. We made the models of impaired spermatogenesis in the SD rats 
      by intraperitoneal injection of CTX and treated the animal models by intragastric 
      administration of MO at the concentrations of 10, 20, 30, and 40 g per kg per d, 
      respectively. After two weeks of medication, we observed the changes in the body 
      weight, testicular and epididymal indexes, and microstructure of the testis 
      tissue, measured the mean seminiferous tubule diameter (MSTD) , and obtained 
      testicular biopsy scores (TBS) in different groups, followed by comparative 
      analyses. RESULTS: After treatment, the CTX + NS group showed no remarkable 
      differences in the body weight ([234.83 +/- 28.77] g) and epididymal index (2.71 +/- 
      0.34) from those of the four CTX + MO groups, but exhibited a significantly lower 
      testicular index ([12.15 +/- 1.04] g) than those in the CTX + 20 g/kg MO ([13.71 +/- 
      0.97] g), CTX + 30 g/kg MO, ([13.30 +/- 0.29] g), and CTX + 40 g/kg MO group 
      ([13.48 +/- 0.51] g) (P < 0.05). Light microscopy revealed obvious pathological 
      changes of the testis tissue in the CTX + NS group and significantly ameliorated 
      structures of the seminiferous tubules in the CTX + MO 10, 20, 30, and 40 g/kg 
      groups, with the MSTD of (204.78 +/- 11.03), (216.55 +/- 10.93), (218.03 +/- 11.23), 
      and (218.59 +/- 14.06) mum, respectively, and the TBS of 9.03 +/- 0.39, 9.69 +/- 0.26, 
      9.83 +/- 0.18, and 9.89 +/- 0.11, respectively, all significantly higher than (189.74 
      +/- 8.55) mum and 5.95 +/- 1.21 in the CTX + NS group (P < 0.05). The efficacy of MO 
      extract was increased in a concentration-dependent manner. CONCLUSION: Morinda 
      officinalis extract can repair cytoxan-induced damage to rat spermatogenesis, 
      with may achieve the best effect at the concentrations of 30 and 40 g per kg per 
      d.
FAU - Chen, Tong-jun
AU  - Chen TJ
FAU - Wang, Wei
AU  - Wang W
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Mutagens)
RN  - 0 (Plant Extracts)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Animals
MH  - Body Weight/drug effects
MH  - Cyclophosphamide/toxicity
MH  - Epididymis/drug effects
MH  - Male
MH  - Morinda/*chemistry
MH  - Mutagens/toxicity
MH  - Plant Extracts/administration & dosage/*pharmacology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/drug effects/pathology
MH  - Spermatogenesis/*drug effects
MH  - Testis/drug effects/ultrastructure
EDAT- 2015/06/30 06:00
MHDA- 2015/09/26 06:00
CRDT- 2015/06/30 06:00
PHST- 2015/06/30 06:00 [entrez]
PHST- 2015/06/30 06:00 [pubmed]
PHST- 2015/09/26 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2015 May;21(5):436-42.