PMID- 26118200
OWN - NLM
STAT- MEDLINE
DCOM- 20150817
LR  - 20191113
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 61
IP  - 5-6
DP  - 2015
TI  - Improved Target Cell Selection and Counting Method for UroVysion Fluorescence in 
      Situ Hybridization.
PG  - 637-42
AB  - BACKGROUND: The UroVysion Bladder Cancer Kit requires morphological analysis of 
      4', 6-diamino-2-phenylindole (DAPI)-stained nuclei to identify target cells for 
      fluorescence in situ hybridization (FISH) signals. Reproducibility and efficiency 
      of target cell selection and counting was evaluated by combining 
      immunofluorescence staining of cytokeratin 7 (CK7) and proliferating cell nuclear 
      antigen (PCNA) with DAPI staining. METHODS: The reactivities to CK7, PCNA, and 
      DAPI were compared between those for different ratios of T24 human bladder 
      carcinoma cells and of cells from the urine of five healthy subjects. Two 
      technicians independently performed five replicate cell counts of urine samples 
      from four bladder cancer patients and one healthy subject. RESULTS: The positive 
      staining rates for CK7 and PCNA were similar to DAPI, but our method showed 
      enhanced inter-observer repeatability and reduced operating time for signal 
      counting. CONCLUSIONS: Our proposed method showed better reproducibility and 
      lesser operational time for signal counting than the DAPI method alone.
FAU - Hattori, Shinichi
AU  - Hattori S
FAU - Kikuchi, Arizumi
AU  - Kikuchi A
FAU - Sawamura, Takahiro
AU  - Sawamura T
FAU - Daimaru, Osami
AU  - Daimaru O
FAU - Horie, Masanobu
AU  - Horie M
FAU - Deguchi, Takashi
AU  - Deguchi T
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
RN  - 0 (Indoles)
RN  - 0 (Keratin-7)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Carcinoma/*diagnosis/urine
MH  - Cell Line, Tumor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Indoles
MH  - Keratin-7/*analysis
MH  - Proliferating Cell Nuclear Antigen/*analysis
MH  - Urinary Bladder Neoplasms/*diagnosis/urine
EDAT- 2015/06/30 06:00
MHDA- 2015/08/19 06:00
CRDT- 2015/06/30 06:00
PHST- 2015/06/30 06:00 [entrez]
PHST- 2015/06/30 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
AID - 10.7754/clin.lab.2014.140903 [doi]
PST - ppublish
SO  - Clin Lab. 2015;61(5-6):637-42. doi: 10.7754/clin.lab.2014.140903.