PMID- 26119274
OWN - NLM
STAT- MEDLINE
DCOM- 20160229
LR  - 20220408
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 199
IP  - 2
DP  - 2015 Dec
TI  - Ultrasound microbubbles enhance human beta-defensin 3 against biofilms.
PG  - 458-69
LID - S0022-4804(15)00610-1 [pii]
LID - 10.1016/j.jss.2015.05.030 [doi]
AB  - BACKGROUND: The infection of orthopedic implantation devices with Staphylococcus 
      has been a serious concern within the biomaterial community. Treatments are not 
      always successful because of antibiotic-resistant bacteria biofilm infection. 
      Recent studies have shown that combination of antibiotics with low-frequency 
      ultrasound (US) can enhance the bactericidal activity effectively against the 
      formation of biofilms in vitro pilot study. Meanwhile, microbubbles evolved as 
      targeted drug-delivery agents can provide nuclei for inertial cavitation and 
      lower the threshold for US-induced cavitation. Human beta-defensin 3 (HBD-3) is a 
      cationic antimicrobial peptide considered particularly promising for future 
      bactericidal employment and has effect on antibiotic-resistant Staphylococcus 
      biofilms. But the effect has not been reported when combined with US-targeted 
      microbubble destruction (UTMD) in vivo. METHODS: In this study, we evaluated the 
      effect of HBD-3 combined with UTMD on two tested Staphylococcus by the spread 
      plate method, crystal violet staining, confocal laser scanning microscopy, 
      scanning electron microscopy, and real-time polymerase chain reaction. RESULTS: 
      In the study, we found that the biofilm densities, the percentage of live cells, 
      and the viable counts of two tested Staphylococcus that recovered from the 
      biofilm on the titanium surface in mice were significantly decreased in the group 
      of the HBD-3 combined with UTMD, compared with those of other groups. 
      Furthermore, in the experiment, we found out that UTMD could enhance HBD-3 
      activity, which inhibits the biofilm-associated genes expression of icaAD and the 
      methicillin-resistance genes expression of MecA by promoting the icaR expression 
      simultaneously. CONCLUSIONS: The combination of HBD-3 with UTMD can play a 
      significant role on the elimination of the antibiotic-resistant Staphylococcus 
      biofilms in vivo.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Li, Shoumin
AU  - Li S
AD  - Department of Orthopaedic Surgery, Anhui Provincial Hospital of Anhui Medical 
      University, Hefei, China.
FAU - Zhu, Chen
AU  - Zhu C
AD  - Department of Orthopaedic Surgery, Anhui Provincial Hospital of Anhui Medical 
      University, Hefei, China.
FAU - Fang, Shiyuan
AU  - Fang S
AD  - Department of Orthopaedic Surgery, Anhui Provincial Hospital of Anhui Medical 
      University, Hefei, China.
FAU - Zhang, Weiwei
AU  - Zhang W
AD  - Department of Geriatrics, Anhui Provincial Hospital of Anhui Medical University, 
      Hefei, China.
FAU - He, Nianan
AU  - He N
AD  - Department of Ultrasound, Anhui Provincial Hospital of Anhui Medical University, 
      Hefei, China.
FAU - Xu, Wei
AU  - Xu W
AD  - Department of Orthopaedic Surgery, Anhui Provincial Hospital of Anhui Medical 
      University, Hefei, China.
FAU - Kong, Rong
AU  - Kong R
AD  - Department of Orthopaedic Surgery, Anhui Provincial Hospital of Anhui Medical 
      University, Hefei, China. Electronic address: kongrongahslyy@163.com.
FAU - Shang, Xifu
AU  - Shang X
AD  - Department of Orthopaedic Surgery, Anhui Provincial Hospital of Anhui Medical 
      University, Hefei, China. Electronic address: shangxifuahslyy@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150522
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Bacterial Proteins)
RN  - 0 (DEFB103A protein, human)
RN  - 0 (MecA protein, Staphylococcus epidermidis)
RN  - 0 (Penicillin-Binding Proteins)
RN  - 0 (beta-Defensins)
RN  - 0 (mecA protein, Staphylococcus aureus)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/metabolism
MH  - *Biofilms
MH  - Drug Evaluation, Preclinical
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microbial Sensitivity Tests
MH  - *Microbubbles
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Penicillin-Binding Proteins
MH  - Phenotype
MH  - Prosthesis-Related Infections/*drug therapy
MH  - Staphylococcus aureus
MH  - Staphylococcus epidermidis
MH  - Titanium
MH  - *Ultrasonic Therapy
MH  - beta-Defensins/*administration & dosage
OTO - NOTNLM
OT  - Biofilm
OT  - Microbubbles
OT  - Mouse
OT  - Staphylococcus
OT  - Ultrasound
OT  - beta-defensin
EDAT- 2015/06/30 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/06/30 06:00
PHST- 2014/10/03 00:00 [received]
PHST- 2015/04/06 00:00 [revised]
PHST- 2015/05/19 00:00 [accepted]
PHST- 2015/06/30 06:00 [entrez]
PHST- 2015/06/30 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - S0022-4804(15)00610-1 [pii]
AID - 10.1016/j.jss.2015.05.030 [doi]
PST - ppublish
SO  - J Surg Res. 2015 Dec;199(2):458-69. doi: 10.1016/j.jss.2015.05.030. Epub 2015 May 
      22.