PMID- 26121872
OWN - NLM
STAT- MEDLINE
DCOM- 20150929
LR  - 20190318
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 46
IP  - 3
DP  - 2015 May
TI  - [Affinity Detection and Quantification of Receptors of TRAIL Based on Their 
      Thermostability].
PG  - 449-52
AB  - OBJECTIVE: To develop a way for tumor necrosis factor-related apoptosis-inducing 
      ligand (TRAIL) receptors quantification in cancer via its' thermostability. 
      METHODS: Endougenous alkaline phosphatase (AP) activity and denaturation 
      temperature of pancreatic cancer cell lines AsPC-1 and Capan-2 were detected. 
      Boiling treated recombinant protein death receptor 5 (DR5), named DR5 AP, as well 
      as pancreatic cancer cells lines AsPC-1 and Capan-2 were incubated with AP-tagged 
      TRAIL (AP-TRAIL), and then reacted with Reagent A and Reagent S, the substrate of 
      AP, to quantitive and in site detection of the receptor. RESULTS: The endougenous 
      AP activity of pancreatic cancer cells lines AsPC-1 and Capan-2 could not be 
      totally inactivated by incubated at 65  degrees C, thus inhibited the detection of TRAIL 
      receptor, but the activity was dramatically decreased after treated with boiling 
      water, whereas the DR5-AP was thermal stable. The surface receptor of AsPC-1 and 
      Capan-2 could be recognized and bound by AP-TRAIL after treated at 100  degrees C, the 
      readings were 2. 210+/-0. 393 and 2. 027+/-0. 019. CONCLUSION: The TRAIL receptors 
      are thermostable and this may provide a better diagnosis and prognosis of cancer 
      as well as personalize cancer therapy.
FAU - Xu, Yang
AU  - Xu Y
FAU - Liu, Hai-peng
AU  - Liu HP
FAU - Long, Tian-zhen
AU  - Long TZ
FAU - Luo, Guang-ping
AU  - Luo GP
FAU - Liang, Peng
AU  - Liang P
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Cell Line, Tumor
MH  - Hot Temperature
MH  - Humans
MH  - Pancreatic Neoplasms/*metabolism
MH  - Protein Stability
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/*metabolism
MH  - Recombinant Proteins/metabolism
EDAT- 2015/07/01 06:00
MHDA- 2015/09/30 06:00
CRDT- 2015/07/01 06:00
PHST- 2015/07/01 06:00 [entrez]
PHST- 2015/07/01 06:00 [pubmed]
PHST- 2015/09/30 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2015 May;46(3):449-52.