PMID- 26122720
OWN - NLM
STAT- MEDLINE
DCOM- 20151117
LR  - 20181202
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 165
DP  - 2015 Aug
TI  - Effects of Anthropogenic Pollution on the Oxidative Phosphorylation Pathway of 
      Hepatocytes from Natural Populations of Fundulus heteroclitus.
PG  - 231-40
LID - S0166-445X(15)00181-2 [pii]
LID - 10.1016/j.aquatox.2015.06.009 [doi]
AB  - Persistent organic pollutants (POPs), including polycyclic aromatic hydrocarbons 
      (PAHs) and polychlorinated biphenyls (PCBs), potentially target mitochondria and 
      cause toxicity. We compared the effects of POPs on mitochondrial respiration by 
      measuring oxidative phosphorylation (OxPhos) metabolism in hepatocytes isolated 
      from lab-depurated Fundulus heteroclitus from a Superfund site contaminated with 
      PAHs (Elizabeth River VA, USA) relative to OxPhos metabolism in individuals from 
      a relatively clean, reference population (King's Creek VA, USA). In individuals 
      from the polluted Elizabeth River population, OxPhos metabolism displayed lower 
      LEAK and lower activities in complex III, complex IV, and E State, but higher 
      activity in complex I compared to individuals from the reference King's Creek 
      population. To test the supposition that these differences were due to or related 
      to the chronic PAH contamination history of the Elizabeth River population, we 
      compared the OxPhos functions of undosed individuals from the polluted and 
      reference populations to individuals from these populations dosed with a PAH 
      benzo [alpha] pyrene (BaP) or a PCB PCB126 (3,3',4,4',5-pentachlorobiphenyl), 
      respectively. Exposure to PAH or PCB affected OxPhos in the reference King's 
      Creek population but had no detectable effects on the polluted Elizabeth River 
      population. Thus, PAH exposure significantly increased LEAK, and exposure to 
      PCB126 significantly decreased State 3, E state and complex I activity in the 
      reference King's Creek population. These data strongly implicate an evolved 
      tolerance in the Elizabeth River fish where dosed fish are not affected by PAH 
      exposure and undosed fish show decreased LEAK and increased State 3 and E state.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Du, Xiao
AU  - Du X
AD  - Rosenstiel School of Marine and Atmospheric Science, 4600 Rickenbacker Causeway, 
      University of Miami, Miami, FL 33149, USA.
FAU - Crawford, Douglas L
AU  - Crawford DL
AD  - Rosenstiel School of Marine and Atmospheric Science, 4600 Rickenbacker Causeway, 
      University of Miami, Miami, FL 33149, USA.
FAU - Oleksiak, Marjorie F
AU  - Oleksiak MF
AD  - Rosenstiel School of Marine and Atmospheric Science, 4600 Rickenbacker Causeway, 
      University of Miami, Miami, FL 33149, USA. Electronic address: 
      moleksiak@rsmas.miami.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150619
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - TSH69IA9XF (3,4,5,3',4'-pentachlorobiphenyl)
SB  - IM
MH  - Animals
MH  - Fundulidae/*physiology
MH  - Hepatocytes/*drug effects
MH  - Oxidative Phosphorylation/*drug effects
MH  - Polychlorinated Biphenyls/toxicity
MH  - Polycyclic Aromatic Hydrocarbons/toxicity
MH  - Rivers
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Benzo [alpha] pyrene
OT  - Elizabeth River
OT  - Fundulus heteroclitus
OT  - Oxidative phosphorylation
OT  - PCB126
EDAT- 2015/07/01 06:00
MHDA- 2015/11/18 06:00
CRDT- 2015/07/01 06:00
PHST- 2015/03/31 00:00 [received]
PHST- 2015/06/11 00:00 [revised]
PHST- 2015/06/12 00:00 [accepted]
PHST- 2015/07/01 06:00 [entrez]
PHST- 2015/07/01 06:00 [pubmed]
PHST- 2015/11/18 06:00 [medline]
AID - S0166-445X(15)00181-2 [pii]
AID - 10.1016/j.aquatox.2015.06.009 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2015 Aug;165:231-40. doi: 10.1016/j.aquatox.2015.06.009. Epub 2015 
      Jun 19.