PMID- 26122733
OWN - NLM
STAT- MEDLINE
DCOM- 20160325
LR  - 20220316
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 120
DP  - 2015 Oct
TI  - Synthesis, characterization and biocompatibility of silver nanoparticles 
      synthesized from Nigella sativa leaf extract in comparison with chemical silver 
      nanoparticles.
PG  - 400-8
LID - S0147-6513(15)00310-3 [pii]
LID - 10.1016/j.ecoenv.2015.06.025 [doi]
AB  - Despite the development potential in the field of nanotechnology, there is a 
      concern about possible effects of nanoparticles on the environment and human 
      health. In this study, silver nanoparticles (AgNPs) were synthesized by 'green' 
      and 'chemical' methods. In the wet-chemistry method, sodium borohydrate, sodium 
      citrate and silver nitrate were used as raw materials. Leaf extract of Nigella 
      sativa was used as reducing as well as capping agent to reduce silver nitrate in 
      the green synthesis method. In addition, toxic responses of both synthesized 
      AgNPs were monitored on bone-building stem cells of mice as well as seed 
      germination and seedling growth of six different plants (Lolium, wheat, bean and 
      common vetch, lettuce and canola). In both synthesis methods, the colorless 
      reaction mixtures turned brown and UV-visible spectra confirmed the presence of 
      silver nanoparticles. Scanning electron microscope (SEM) observations revealed 
      the predominance of silver nanosized crystallites and fourier transform infra-red 
      spectroscopy (FTIR) indicated the role of different functional groups in the 
      synthetic process. MTT assay showed cell viability of bone-building stem cells of 
      mice was further in the green AgNPs synthesized using black cumin extract than 
      chemical AgNPs. IC50 (inhibitory concentrations) values for seed germination, 
      root and shoot length for 6 plants in green AgNPs exposures were higher than the 
      chemical AgNPs. These results suggest that cytotoxicity and phytotoxicity of the 
      green synthesized AgNPs were significantly less than wet-chemistry synthesized 
      ones. This study indicated an economical, simple and efficient ecofriendly 
      technique using leaves of N. sativa for synthesis of AgNPs and confirmed that 
      green AgNPs are safer than chemically-synthesized AgNPs.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Amooaghaie, Rayhaneh
AU  - Amooaghaie R
AD  - Department of Biology, Shahrekord University, Shahrekord, Iran. Electronic 
      address: Rayhanehamooaghaie@yahoo.com.
FAU - Saeri, Mohammad Reza
AU  - Saeri MR
AD  - Department of Material Engineering, Shahrekord University, Shahrekord, Iran. 
      Electronic address: Saeri_mohammad@yahoo.com.
FAU - Azizi, Morteza
AU  - Azizi M
AD  - Department of Material Engineering, Shahrekord University, Shahrekord, Iran. 
      Electronic address: morteza2105@yahoo.com.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150626
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Biocompatible Materials)
RN  - 0 (Insecticides)
RN  - 0 (Plant Extracts)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Inhibitory Concentration 50
MH  - Insecticides/analysis
MH  - Larva/drug effects
MH  - Metal Nanoparticles/*chemistry
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Nigella sativa/*chemistry
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves/*chemistry
MH  - Silver/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - Green synthesis
OT  - Nano particle
OT  - Nigella sativa
OT  - Silver
OT  - Toxicity
EDAT- 2015/07/01 06:00
MHDA- 2016/03/26 06:00
CRDT- 2015/07/01 06:00
PHST- 2015/02/02 00:00 [received]
PHST- 2015/06/13 00:00 [revised]
PHST- 2015/06/15 00:00 [accepted]
PHST- 2015/07/01 06:00 [entrez]
PHST- 2015/07/01 06:00 [pubmed]
PHST- 2016/03/26 06:00 [medline]
AID - S0147-6513(15)00310-3 [pii]
AID - 10.1016/j.ecoenv.2015.06.025 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2015 Oct;120:400-8. doi: 10.1016/j.ecoenv.2015.06.025. 
      Epub 2015 Jun 26.