PMID- 26123291
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20200930
IS  - 1432-203X (Electronic)
IS  - 0721-7714 (Linking)
VI  - 34
IP  - 9
DP  - 2015 Sep
TI  - FISH and GISH: molecular cytogenetic tools and their applications in ornamental 
      plants.
PG  - 1477-88
LID - 10.1007/s00299-015-1828-3 [doi]
AB  - The innovations in chromosome engineering have improved the efficiency of 
      interrogation breeding, and the identification and transfer of resistance genes 
      from alien to native species. Recent advances in molecular biology and 
      cytogenetics have brought revolutionary, conceptual developments in mitosis and 
      meiosis research, chromosome structure and manipulation, gene expression and 
      regulation, and gene silencing. Cytogenetic studies offer integrative tools for 
      imaging, genetics, epigenetics, and cytological information that can be employed 
      to enhance chromosome and molecular genomic research in plant taxa. In situ 
      hybridization techniques, such as fluorescence in situ hybridization (FISH) and 
      genomic in situ hybridization (GISH), can identify chromosome morphologies and 
      sequences, amount and distribution of various types of chromatin in chromosomes, 
      and genome organization during the metaphase stage of meiosis. Over the past few 
      decades, various new molecular cytogenetic applications have been developed. The 
      FISH and GISH techniques present an authentic model for analyzing the individual 
      chromosome, chromosomal segments, or the genomes of natural and artificial hybrid 
      plants. These have become the most reliable techniques for studying 
      allopolyploids, because most cultivated plants have been developed through 
      hybridization or polyploidization. Moreover, introgression of the genes and 
      chromatin from the wild types into cultivated species can also be analyzed. Since 
      hybrid derivatives may have variable alien chromosome numbers or chromosome arms, 
      the use of these approaches opens new avenues for accurately identifying genome 
      differences.
FAU - Younis, Adnan
AU  - Younis A
AD  - Department of Horticultural Science, Kyungpook National University, Daegu, 
      702-701, South Korea, adnan@knu.ac.kr.
FAU - Ramzan, Fahad
AU  - Ramzan F
FAU - Hwang, Yoon-Jung
AU  - Hwang YJ
FAU - Lim, Ki-Byung
AU  - Lim KB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150630
PL  - Germany
TA  - Plant Cell Rep
JT  - Plant cell reports
JID - 9880970
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - Cytogenetic Analysis/*methods
MH  - *Genome, Plant
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Plants/*genetics
MH  - Recombination, Genetic/genetics
EDAT- 2015/07/01 06:00
MHDA- 2016/05/10 06:00
CRDT- 2015/07/01 06:00
PHST- 2015/05/23 00:00 [received]
PHST- 2015/06/15 00:00 [accepted]
PHST- 2015/07/01 06:00 [entrez]
PHST- 2015/07/01 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
AID - 10.1007/s00299-015-1828-3 [doi]
PST - ppublish
SO  - Plant Cell Rep. 2015 Sep;34(9):1477-88. doi: 10.1007/s00299-015-1828-3. Epub 2015 
      Jun 30.