PMID- 26124680
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150630
LR  - 20240515
IS  - 1179-0660 (Print)
IS  - 1179-0660 (Electronic)
IS  - 1179-0660 (Linking)
VI  - 2
DP  - 2009
TI  - A Novel High-Throughput Technique for Identifying Monoclonal Antibodies Capable 
      of Death Receptor Induced Apoptosis.
PG  - 45-51
AB  - The study of death receptor family induced apoptosis has gained momentum in 
      recent years with the knowledge that therapeutic antibodies targeting DR4 and DR5 
      (death receptor's 4 and 5) have proved efficacious in multiple clinical trials. 
      The therapeutic rationale is based on targeting and amplifying a tumour tissues 
      normal cell death programme (apoptosis). While advances in the targeting of DR4 
      and DR5 have been successful the search for an agonistic antibody to another 
      family member, the Fas receptor, has proven more elusive. This is partly due to 
      the differing in vitro and in vivo characteristics of individual antibodies. In 
      order to induce Fas targeted cell death an antibody must be capable of binding to 
      and trimerising the receptor. It has been shown that antibodies capable of 
      performing this function in vivo, with the assistance of tumour associated cells, 
      do not always induce apoptosis in vitro. As a result the use of current 
      methodologies to detect functional antibodies in vitro may have dismissed 
      potential therapeutic candidates ('false negative'). Here we report a novel high 
      throughput screening technique which artificially cross-links antibodies bound to 
      the Fas receptor. By combining this process with Annexin-V and Prodidium Iodide 
      (PI) staining we can select for antibodies which have the potential to induce 
      apoptosis in vivo.
FAU - Kwok, Hang Fai
AU  - Kwok HF
AD  - Fusion Antibodies Ltd., Springbank Ind. Est. Belfast, Northern Ireland BT17 0QL.
FAU - Gormley, Julie A
AU  - Gormley JA
AD  - Fusion Antibodies Ltd., Springbank Ind. Est. Belfast, Northern Ireland BT17 0QL.
FAU - Scott, Christopher J
AU  - Scott CJ
AD  - School of Pharmacy, Queen's University Belfast, Northern Ireland BT9 7BL.
FAU - Johnston, James A
AU  - Johnston JA
AD  - Fusion Antibodies Ltd., Springbank Ind. Est. Belfast, Northern Ireland BT17 0QL.
FAU - Olwill, Shane A
AU  - Olwill SA
AD  - Fusion Antibodies Ltd., Springbank Ind. Est. Belfast, Northern Ireland BT17 0QL.
LA  - eng
PT  - Journal Article
DEP - 20091202
PL  - United States
TA  - J Cell Death
JT  - Journal of cell death
JID - 101534028
PMC - PMC4474333
OTO - NOTNLM
OT  - annexin-V
OT  - apoptosis
OT  - crosslinks antibodies
OT  - fas receptor
OT  - high throughput screening
OT  - prodidium iodide
OT  - therapeutic antibodies
EDAT- 2009/01/01 00:00
MHDA- 2009/01/01 00:01
PMCR- 2009/12/02
CRDT- 2015/07/01 06:00
PHST- 2015/07/01 06:00 [entrez]
PHST- 2009/01/01 00:00 [pubmed]
PHST- 2009/01/01 00:01 [medline]
PHST- 2009/12/02 00:00 [pmc-release]
AID - jcd-2-2009-045 [pii]
AID - 10.4137/jcd.s3660 [doi]
PST - epublish
SO  - J Cell Death. 2009 Dec 2;2:45-51. doi: 10.4137/jcd.s3660. eCollection 2009.