PMID- 26125786
OWN - NLM
STAT- MEDLINE
DCOM- 20160412
LR  - 20150701
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 2
DP  - 2015 Jun 1
TI  - Assessment of genetic diversity in lentils (Lens culinaris Medik.) based on SNPs.
PG  - 5870-8
LID - 10.4238/2015.June.1.4 [doi]
AB  - This study is the first attempt to establish an SNP database for the purpose of 
      estimating the genetic diversity and relatedness of Palestinian lentil genotypes. 
      A total of 14 lentil accessions (11 local, two supplied by ICARDA, and one 
      introduced from Italy) were investigated. By sequencing two genes, lectin and 
      lipid transfer protein 5 (LTP5), four SNPs were detected (three in the first and 
      one in latter gene) with average frequencies of one SNP every 228 and 578 bp, 
      respectively. In addition, in LTP5 two single-nucleotide indels were observed in 
      the non-coding part of the gene. Four haplotypes were identified in the lectin 
      gene, three in LTP5. One lectin haplotype coincided with that present in GenBank 
      belonging to two cultivated varieties, two were rather similar to this, whereas 
      the last one turned out closer to the sequence of one wild lentil accession, 
      indicating the existence of diversity in the Palestinian germplasm. These 
      results, enhancing the available knowledge of lentil genetic resources in 
      Palestine, may contribute to their conservation and utilization in breeding 
      projects.
FAU - Basheer-Salimia, R
AU  - Basheer-Salimia R
AD  - Department of Plant Production and Protection, Faculty of Agriculture, Hebron 
      University, Hebron, Palestine.
FAU - Camilli, B
AU  - Camilli B
AD  - Overseas Onlus, Spilamberto, Modena, Italy.
FAU - Scacchi, S
AU  - Scacchi S
AD  - Department of Agricultural Sciences, Alma Mater Studiorum University of Bologna, 
      Bologna, Italy.
FAU - Noli, E
AU  - Noli E
AD  - Affiliated Cancer Hospital of Guangxi Medical University, Nanning, China.
FAU - Awad, M
AU  - Awad M
AD  - Department of Plant Production and Protection, Faculty of Agriculture, Hebron 
      University, Hebron, Palestine.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150601
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
SB  - IM
MH  - Breeding
MH  - *Genetic Variation
MH  - *Genotype
MH  - Haplotypes
MH  - INDEL Mutation/genetics
MH  - Italy
MH  - Lens Plant/*genetics
MH  - *Phylogeny
MH  - Polymorphism, Single Nucleotide
MH  - Sequence Analysis, DNA
EDAT- 2015/07/01 06:00
MHDA- 2016/04/14 06:00
CRDT- 2015/07/01 06:00
PHST- 2015/07/01 06:00 [entrez]
PHST- 2015/07/01 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
AID - gmr4986 [pii]
AID - 10.4238/2015.June.1.4 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Jun 1;14(2):5870-8. doi: 10.4238/2015.June.1.4.