PMID- 26125792
OWN - NLM
STAT- MEDLINE
DCOM- 20160412
LR  - 20150701
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 2
DP  - 2015 Jun 1
TI  - Molecular cloning, characterization and expression analysis of a Doublesex gene 
      from Daphnia carinata (Crustacea: Cladocera) during different reproductive 
      stages.
PG  - 5930-842
LID - 10.4238/2015.June.1.10 [doi]
AB  - To better understand the reproductive transformation mechanism of Daphnia 
      carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA 
      ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and 
      assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, 
      and 85.11% identity to orthologous genes in D. magna, D. pulex, and D. galeata 
      respectively. Phylogenetic analysis revealed that DapcaDsx2 clustered in the same 
      class, and was evolutionarily more distant to sequences from other species. 
      qRT-PCR showed that DapcaDsx2 was most abundantly expressed during sexual 
      reproduction (P < 0.05). Using digoxigenin-labeled RNA probes, we studied 
      DapcaDsx2 expression in parthenogenetic and sexual females by whole-mount in situ 
      hybridization. The results revealed that DapcaDsx2 was mainly expressed in the 
      second antennae and several sites of the ventral carapace, whereas higher 
      expression levels were found in sexual than in parthenogenetic females. This 
      suggests that the DapcaDsx2 gene is involved in switching modes of reproduction 
      and in sexual differentiation.
FAU - Zhang, M Q
AU  - Zhang MQ
AD  - School of Life Science, East China Normal University, Shanghai, China.
FAU - Ma, C A
AU  - Ma CA
AD  - School of Life Science, East China Normal University, Shanghai, China.
FAU - Lv, W W
AU  - Lv WW
AD  - School of Life Science, East China Normal University, Shanghai, China.
FAU - Huang, Y H
AU  - Huang YH
AD  - School of Life Science, East China Normal University, Shanghai, China.
FAU - Wang, D L
AU  - Wang DL
AD  - School of Marine Sciences, Ningbo University, Ningbo, China.
FAU - Zhao, Y L
AU  - Zhao YL
AD  - School of Life Science, East China Normal University, Shanghai, China 
      zhaoyunlong_ecnu@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150601
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Arthropod Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DSX protein, Drosophila)
RN  - 0 (Drosophila Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arthropod Proteins/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - DNA-Binding Proteins/genetics
MH  - Daphnia/*genetics/growth & development
MH  - Drosophila Proteins/genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Parthenogenesis/*genetics
MH  - *Phylogeny
MH  - Reproduction/*genetics
EDAT- 2015/07/01 06:00
MHDA- 2016/04/14 06:00
CRDT- 2015/07/01 06:00
PHST- 2015/07/01 06:00 [entrez]
PHST- 2015/07/01 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
AID - gmr5279 [pii]
AID - 10.4238/2015.June.1.10 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Jun 1;14(2):5930-842. doi: 10.4238/2015.June.1.10.