PMID- 26125893
OWN - NLM
STAT- MEDLINE
DCOM- 20160325
LR  - 20220330
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 2
DP  - 2015 Jun 18
TI  - Expression of TNF-alpha, VEGF, and MMP-3 mRNAs in synovial tissues and their roles in 
      fibroblast-mediated osteogenesis in ankylosing spondylitis.
PG  - 6852-8
LID - 10.4238/2015.June.18.28 [doi]
AB  - The aim of this study was to explore the mRNA levels of tumor necrosis factor-alpha 
      (TNF-alpha), vessel endothelial growth factor (VEGF), and matrix metalloproteinase-3 
      (MMP-3) in synovial tissues in ankylosing spondylitis (AS), and to analyze the 
      functions of these proteins in the differentiation of AS synovial tissue 
      fibroblasts into osteoblasts (OB) and osteoclasts. Synovial tissue samples from 
      22 AS patients and 22 normal individuals were collected. In situ hybridization 
      was utilized to detect TNF-alpha, VEGF, and MMP-3 transcripts. After counting numbers 
      of positive cells, Spearman analysis was used to determine the correlation 
      between transcriptional levels of the three mRNAs and the AS disease activity 
      index (BASDAI) and the C-response protein (CRP) levels. With the addition of 
      TNF-alpha, VEGF, or both factors into cultured normal synovial fibroblasts, 
      osteocalcin (bone gla protein, BGP) secretion levels were compared. We found that 
      expression of TNF-alpha, VEGF, and MMP-3 was identified exclusively in the disease 
      group. mRNA levels were significantly positively correlated with BASDAI (r = 
      0.42, 0.38, and 0.47, respectively; P < 0.05) and CRP (r = 0.44, 0.34, and 0.47 
      respectively; P < 0.05) scores. The secretion level of BGP in normal synovial 
      fibroblasts increased progressively with increasing concentrations of VEGF or 
      TNF-alpha (P < 0.01 compared to levels before treatment). Furthermore, co-incubation 
      using both VEGF and TNF-alpha significantly elevated BGP levels compared to the 
      single addition of VEGF or TNF-alpha (P < 0.01). These results suggest TNF-alpha, VEGF, 
      and MMP-3 might directly participate in the differentiation of fibroblasts into 
      OBs.
FAU - Liu, K G
AU  - Liu KG
AD  - Department of Orthopaedic Surgery, Yantaishan Hospital, Yantai, Shandong, China.
FAU - He, Q H
AU  - He QH
AD  - Department of Orthopaedic Surgery, Yantaishan Hospital, Yantai, Shandong, China.
FAU - Tan, J W
AU  - Tan JW
AD  - Department of Orthopaedic Surgery, Yantaishan Hospital, Yantai, Shandong, China.
FAU - Liao, G J
AU  - Liao GJ
AD  - Department of Orthopaedic Surgery, Yantaishan Hospital, Yantai, Shandong, China 
      ytlgj2004as@163.com.
LA  - eng
PT  - Journal Article
DEP - 20150618
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 104982-03-8 (Osteocalcin)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - C-Reactive Protein/genetics/metabolism
MH  - Case-Control Studies
MH  - Cell Differentiation
MH  - Female
MH  - Fibroblasts/*metabolism/pathology
MH  - Gene Expression
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Osteoblasts/*metabolism/pathology
MH  - Osteocalcin/genetics/metabolism
MH  - Osteoclasts/*metabolism/pathology
MH  - Osteogenesis/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Spondylitis, Ankylosing/*genetics/metabolism/pathology
MH  - Synovial Membrane/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
EDAT- 2015/07/01 06:00
MHDA- 2016/03/26 06:00
CRDT- 2015/07/01 06:00
PHST- 2015/07/01 06:00 [entrez]
PHST- 2015/07/01 06:00 [pubmed]
PHST- 2016/03/26 06:00 [medline]
AID - gmr5492 [pii]
AID - 10.4238/2015.June.18.28 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Jun 18;14(2):6852-8. doi: 10.4238/2015.June.18.28.