PMID- 26129933
OWN - NLM
STAT- MEDLINE
DCOM- 20160518
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 3
DP  - 2015 Sep
TI  - Characteristics of human umbilical cord mesenchymal stem cells during ex vivo 
      expansion.
PG  - 4320-4325
LID - 10.3892/mmr.2015.3999 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) have potential clinical 
      applications in different types of diseases. In order to acquire enough cells, 
      hUCMSCs have to be expanded ex vivo. However, it remains to be elucidated whether 
      the characteristics of hUCMSCs are altered during ex vivo expansion. In the 
      present study, the quality of hUCMSCs, which is important for successful 
      therapeutic use, was systematically examined during hUCMSC expansion ex vivo. 
      Morphologically, hUCMSCs exhibited no visible changes during culture. In 
      addition, hUCMSCs retained their proliferative ability between passages 0-5. At 
      the molecular level, the cells continued expressing the specific positive surface 
      markers, CD29, CD73 and CD90, and did not express the negative surface markers, 
      CD14, CD34 or CD45, during culture ex vivo. Furthermore, the hUCMSCs exhibited 
      low immunogenicity, which was maintained when cultured for five passages. 
      However, the immunological properties of hUCMSCs were altered at passage 10, at 
      which the percentage of hUCMSCs expressing human leukocyte antigen‑I was 
      significantly increased. Collectively, these results suggested that hUCMSCs used 
      for cell-based therapies require obtaining from cells, which have been expanded 
      for fewer than five passages.
FAU - Li, Shengying
AU  - Li S
AD  - Department of Pharmacology, Xinxiang Medical University, Xinxiang, Henan 453003, 
      P.R. China.
FAU - Wang, Yuxia
AU  - Wang Y
AD  - Department of Pathophysiology, Xinxiang Medical University, Xinxiang, Henan 
      453003, P.R. China.
FAU - Guan, Liping
AU  - Guan L
AD  - Department of Functional Science Laboratory, Xinxiang Medical University, 
      Xinxiang, Henan 453003, P.R. China.
FAU - Ji, Mingli
AU  - Ji M
AD  - Department of Physiology, Xinxiang Medical University, Xinxiang, Henan 453003, 
      P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150625
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antigens, CD)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Shape
MH  - Cells, Cultured
MH  - HLA Antigens/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*physiology
MH  - Umbilical Cord/cytology
EDAT- 2015/07/02 06:00
MHDA- 2016/05/19 06:00
CRDT- 2015/07/02 06:00
PHST- 2014/05/15 00:00 [received]
PHST- 2015/01/30 00:00 [accepted]
PHST- 2015/07/02 06:00 [entrez]
PHST- 2015/07/02 06:00 [pubmed]
PHST- 2016/05/19 06:00 [medline]
AID - 10.3892/mmr.2015.3999 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Sep;12(3):4320-4325. doi: 10.3892/mmr.2015.3999. Epub 2015 Jun 
      25.