PMID- 26131203
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150701
LR  - 20220330
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 4
DP  - 2015
TI  - Repair of osteochondral defects by mosaicplasty and allogeneic BMSCs 
      transplantation.
PG  - 6053-9
AB  - OBJECTIVE: To investigate the feasibility and efficacy of repairing osteochondral 
      defects with mosaicplasty and allogeneic bone marrow mesenchymal stem cells 
      (BMSCs) transplantation. METHODS: BMSCs were harvested from rabbits and 
      maintained in vitro. Cells of third passage were mixed with pluronic F-127. 
      Osteochondral defect animal model was established in rabbits and then this defect 
      was treated with autologous osteochondral grafts with or without BMSCs above 
      mentioned. In control group, pure pluronic F-127 was filled in the defect. 
      Histological and immunohistological examinations were performed for the 
      evaluation of therapeutic effectiveness. RESULTS: Autologous osteochondral grafts 
      in both groups were not loose, prolapsed and depressed. In BMSCs group, the 
      tissues in the "death space" became hyaline cartilage. The arrangement of 
      chondrocytes was regular. At 4, 8, 12 and 16 weeks, O'Driscoll and Keeley and 
      Salter score were 14.00+/-1.00, 16.75+/-1.71, 18.00+/-0.82 and 20.50+/-1.29 in BMSCs 
      group, which were significantly higher than those in control group (7.67+/-0.58, 
      8.00+/-0.82, 8.50+/-0.58 and 9.00+/-0.82, respectively). There were significant 
      differences among different treatments (F=584.028, P=0.000), but the score was 
      comparable between right defect and left defect (F=0.028, P=0.890). In addition, 
      significant difference was also observed at different time points (F=18.364, 
      P=0.000), but there was no interaction between time and treatment (F=6.939, 
      P=0.015). Moreover, interactions among other factors were also not observed. 
      CONCLUSION: Mosaicplasty and BMSC transplantation are effective to repair the 
      osteochondral defects and integrate the "death space", achieving a better 
      therapeutic efficacy. Thus, this combined therapy may become an effective 
      strategy for the therapy of osteochondral defects.
FAU - Ma, Xin
AU  - Ma X
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Sun, Yuan
AU  - Sun Y
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Cheng, Xiangguo
AU  - Cheng X
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Gao, Youshui
AU  - Gao Y
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Hu, Bin
AU  - Hu B
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Wen, Gen
AU  - Wen G
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Qian, Yebin
AU  - Qian Y
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Gu, Wenqi
AU  - Gu W
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Mao, Yanjie
AU  - Mao Y
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
FAU - Liu, Wanjun
AU  - Liu W
AD  - Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital Shanghai 200233, China.
LA  - eng
PT  - Journal Article
DEP - 20150415
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4483996
OTO - NOTNLM
OT  - Allograft
OT  - bone marrow mesenchymal stem cells
OT  - mosaicplasty
OT  - osteochondral defect
EDAT- 2015/07/02 06:00
MHDA- 2015/07/02 06:01
PMCR- 2015/04/15
CRDT- 2015/07/02 06:00
PHST- 2015/02/02 00:00 [received]
PHST- 2015/04/03 00:00 [accepted]
PHST- 2015/07/02 06:00 [entrez]
PHST- 2015/07/02 06:00 [pubmed]
PHST- 2015/07/02 06:01 [medline]
PHST- 2015/04/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Apr 15;8(4):6053-9. eCollection 2015.