PMID- 26132316
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150703
LR  - 20220331
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 7
IP  - 3
DP  - 2015 Jun 26
TI  - High-Fat, High-Calorie Diet Enhances Mammary Carcinogenesis and Local 
      Inflammation in MMTV-PyMT Mouse Model of Breast Cancer.
PG  - 1125-42
LID - 10.3390/cancers7030828 [doi]
AB  - Epidemiological studies provide strong evidence that obesity and the associated 
      adipose tissue inflammation are risk factors for breast cancer; however, the 
      molecular mechanisms are poorly understood. We evaluated the effect of a 
      high-fat/high-calorie diet on mammary carcinogenesis in the immunocompetent 
      MMTV-PyMT murine model. Four-week old female mice (20/group) were randomized to 
      receive either a high-fat (HF; 60% kcal as fat) or a low-fat (LF; 16% kcal) diet 
      for eight weeks. Body weights were determined, and tumor volumes measured by 
      ultrasound, each week. At necropsy, the tumors and abdominal visceral fat were 
      weighed and plasma collected. The primary mammary tumors, adjacent mammary fat, 
      and lungs were preserved for histological and immunohistochemical examination and 
      quantification of infiltrating macrophages, crown-like structure (CLS) formation, 
      and microvessel density. The body weight gains, visceral fat weights, the primary 
      mammary tumor growth rates and terminal weights, were all significantly greater 
      in the HF-fed mice. Adipose tissue inflammation in the HF group was indicated by 
      hepatic steatosis, pronounced macrophage infiltration and CLS formation, and 
      elevations in plasma monocyte chemoattractant protein-1 (MCP-1), leptin and 
      proinflammatory cytokine concentrations. HF intake was also associated with 
      higher tumor-associated microvascular density and the proangiogenic factor MCP-1. 
      This study provides preclinical evidence in a spontaneous model of breast cancer 
      that mammary adipose tissue inflammation induced by diet, enhances the 
      recruitment of macrophages and increases tumor vascular density suggesting a role 
      for obesity in creating a microenvironment favorable for angiogenesis in the 
      progression of breast cancer.
FAU - Cowen, Sarah
AU  - Cowen S
AD  - Department of Surgery, West Virginia University Health Sciences Center, 
      Morgantown, WV 26506, USA. scowen@miis.edu.
AD  - Mary Babb Randolph Cancer Center, West Virginia University Health Sciences 
      Center, Morgantown, WV 26506, USA. scowen@miis.edu.
FAU - McLaughlin, Sarah L
AU  - McLaughlin SL
AD  - Mary Babb Randolph Cancer Center, West Virginia University Health Sciences 
      Center, Morgantown, WV 26506, USA. smclaughlin@hsc.wvu.edu.
FAU - Hobbs, Gerald
AU  - Hobbs G
AD  - Mary Babb Randolph Cancer Center, West Virginia University Health Sciences 
      Center, Morgantown, WV 26506, USA. ghobbs@stat.wvu.edu.
AD  - Department of Statistics, West Virginia University, Morgantown, WV 26506, USA. 
      ghobbs@stat.wvu.edu.
FAU - Coad, James
AU  - Coad J
AD  - Department of Pathology, West Virginia University Health Sciences Center, 
      Morgantown, WV 26506, USA. jcoad@hsc.wvu.edu.
FAU - Martin, Karen H
AU  - Martin KH
AD  - Mary Babb Randolph Cancer Center, West Virginia University Health Sciences 
      Center, Morgantown, WV 26506, USA. kamartin@hsc.wvu.edu.
AD  - Department of Neurobiology and Anatomy, West Virginia University Health Sciences 
      Center, Morgantown, WV 26506, USA. kamartin@hsc.wvu.edu.
FAU - Olfert, I Mark
AU  - Olfert IM
AD  - Mary Babb Randolph Cancer Center, West Virginia University Health Sciences 
      Center, Morgantown, WV 26506, USA. imolfert@hsc.wvu.edu.
AD  - Department of Human Performance and Exercise Physiology, West Virginia University 
      Health Sciences Center, Morgantown, WV 26506, USA. imolfert@hsc.wvu.edu.
FAU - Vona-Davis, Linda
AU  - Vona-Davis L
AD  - Department of Surgery, West Virginia University Health Sciences Center, 
      Morgantown, WV 26506, USA. lvdavis@hsc.wvu.edu.
AD  - Mary Babb Randolph Cancer Center, West Virginia University Health Sciences 
      Center, Morgantown, WV 26506, USA. lvdavis@hsc.wvu.edu.
LA  - eng
GR  - P20 RR016440/RR/NCRR NIH HHS/United States
GR  - P30 RR032138/RR/NCRR NIH HHS/United States
GR  - U54 GM104942/GM/NIGMS NIH HHS/United States
GR  - P20 RR016477/RR/NCRR NIH HHS/United States
GR  - S10 RR026378/RR/NCRR NIH HHS/United States
GR  - P20 GM103434/GM/NIGMS NIH HHS/United States
GR  - P30 GM103488/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20150626
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC4586761
OTO - NOTNLM
OT  - angiogenesis
OT  - high-fat diet
OT  - inflammation
OT  - obesity
OT  - tumor progression
EDAT- 2015/07/02 06:00
MHDA- 2015/07/02 06:01
PMCR- 2015/06/26
CRDT- 2015/07/02 06:00
PHST- 2015/05/15 00:00 [received]
PHST- 2015/06/16 00:00 [revised]
PHST- 2015/06/18 00:00 [accepted]
PHST- 2015/07/02 06:00 [entrez]
PHST- 2015/07/02 06:00 [pubmed]
PHST- 2015/07/02 06:01 [medline]
PHST- 2015/06/26 00:00 [pmc-release]
AID - cancers7030828 [pii]
AID - cancers-07-00828 [pii]
AID - 10.3390/cancers7030828 [doi]
PST - epublish
SO  - Cancers (Basel). 2015 Jun 26;7(3):1125-42. doi: 10.3390/cancers7030828.