PMID- 26133983
OWN - NLM
STAT- MEDLINE
DCOM- 20160419
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 7
DP  - 2015
TI  - The Porcine TSPY Gene Is Tricopy but Not a Copy Number Variant.
PG  - e0131745
LID - 10.1371/journal.pone.0131745 [doi]
LID - e0131745
AB  - The testis-specific protein Y-encoded (TSPY) gene is situated on the mammalian 
      Y-chromosome and exhibits some remarkable biological characteristics. It has the 
      highest known copy number (CN) of all protein coding genes in the human and 
      bovine genomes (up to 74 and 200, respectively) and also shows high individual 
      variability. Although the biological function of TSPY has not yet been 
      elucidated, its specific expression in the testis and several identified binding 
      domains within the protein suggests roles in male reproduction. Here we describe 
      the porcine TSPY, as a multicopy gene with three copies located on the short arm 
      of the Y-chromosome with no variation at three exon loci among 20 animals of 
      normal reproductive health from four breeds of domestic pigs (Pietrain, Landrace, 
      Duroc and Yorkshire). To further investigate the speculation that porcine TSPY is 
      not a copy number variant, we have included five Low-fertility boars and five 
      boars with exceptional High-fertility records. Interestingly, there was no 
      difference between the High- and Low-fertile groups, but we detected slightly 
      lower TSPY CN at all three exons (2.56-2.85) in both groups, as compared to 
      normal animals, which could be attributed to technical variability or somatic 
      mosaicism. The results are based on both relative quantitative real-time PCR 
      (qPCR) and droplet digital PCR (ddPCR). Chromosomal localization of the porcine 
      TSPY was done using fluorescence in situ hybridization (FISH) with gene specific 
      PCR probes.
FAU - Quach, Anh T
AU  - Quach AT
AD  - Department of Biomedical Sciences, Ontario Veterinary College, University of 
      Guelph, Guelph, Ontario, Canada.
FAU - Oluwole, Olutobi
AU  - Oluwole O
AD  - Department of Biomedical Sciences, Ontario Veterinary College, University of 
      Guelph, Guelph, Ontario, Canada.
FAU - King, William Allan
AU  - King WA
AD  - Department of Biomedical Sciences, Ontario Veterinary College, University of 
      Guelph, Guelph, Ontario, Canada.
FAU - Revay, Tamas
AU  - Revay T
AD  - Department of Biomedical Sciences, Ontario Veterinary College, University of 
      Guelph, Guelph, Ontario, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150702
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cell Cycle Proteins)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/*genetics
MH  - *DNA Copy Number Variations
MH  - Exons
MH  - Female
MH  - Fertility
MH  - Gene Dosage
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Real-Time Polymerase Chain Reaction
MH  - Swine
MH  - Temperature
MH  - Testis/metabolism
PMC - PMC4489747
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/07/03 06:00
MHDA- 2016/04/20 06:00
PMCR- 2015/07/02
CRDT- 2015/07/03 06:00
PHST- 2014/12/30 00:00 [received]
PHST- 2015/06/05 00:00 [accepted]
PHST- 2015/07/03 06:00 [entrez]
PHST- 2015/07/03 06:00 [pubmed]
PHST- 2016/04/20 06:00 [medline]
PHST- 2015/07/02 00:00 [pmc-release]
AID - PONE-D-14-57662 [pii]
AID - 10.1371/journal.pone.0131745 [doi]
PST - epublish
SO  - PLoS One. 2015 Jul 2;10(7):e0131745. doi: 10.1371/journal.pone.0131745. 
      eCollection 2015.