PMID- 26134672 OWN - NLM STAT- MEDLINE DCOM- 20160324 LR - 20181113 IS - 1471-2156 (Electronic) IS - 1471-2156 (Linking) VI - 16 DP - 2015 Jul 2 TI - High resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of Rosa. PG - 74 LID - 10.1186/s12863-015-0233-9 [doi] LID - 74 AB - BACKGROUND: Rosaceae is a family containing many economically important fruit and ornamental species. Although fluorescence in situ hybridization (FISH)-based physical mapping of plant genomes is a valuable tool for map-based cloning, comparative genomics and evolutionary studies, no studies using high resolution physical mapping have been performed in this family. Previously we proved that physical mapping of single-copy genes as small as 1.1 kb is possible on mitotic metaphase chromosomes of Rosa wichurana using Tyramide-FISH. In this study we aimed to further improve the physical map of Rosa wichurana by applying high resolution FISH to pachytene chromosomes. RESULTS: Using high resolution Tyramide-FISH and multicolor Tyramide-FISH, 7 genes (1.7-3 kb) were successfully mapped on pachytene chromosomes 4 and 7 of Rosa wichurana. Additionally, by using multicolor Tyramide-FISH three closely located genes were simultaneously visualized on chromosome 7. A detailed map of heterochromatine/euchromatine patterns of chromosome 4 and 7 was developed with indication of the physical position of these 7 genes. Comparison of the gene order between Rosa wichurana and Fragaria vesca revealed a poor collinearity for chromosome 7, but a perfect collinearity for chromosome 4. CONCLUSIONS: High resolution physical mapping of short probes on pachytene chromosomes of Rosa wichurana was successfully performed for the first time. Application of Tyramide-FISH on pachytene chromosomes allowed the mapping resolution to be increased up to 20 times compared to mitotic metaphase chromosomes. High resolution Tyramide-FISH and multicolor Tyramide-FISH might become useful tools for further physical mapping of single-copy genes and for the integration of physical and genetic maps of Rosa wichurana and other members of the Rosaceae. FAU - Kirov, Ilya V AU - Kirov IV AD - Department of Genetics, Biotechnology, Plant Breeding and Seed Science, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Timiryazevskay str.49, 127550, Moscow, Russia. kirovez@gmail.com. AD - Center of Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Listvennichnaya Alleya 5, 127550, Moscow, Russia. kirovez@gmail.com. AD - Plant Sciences Unit, Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research (ILVO), Caritasstraat 21, 9090, Melle, Belgium. kirovez@gmail.com. FAU - Van Laere, Katrijn AU - Van Laere K AD - Plant Sciences Unit, Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research (ILVO), Caritasstraat 21, 9090, Melle, Belgium. Katrijn.Vanlaere@ilvo.vlaanderen.be. FAU - Khrustaleva, Ludmila I AU - Khrustaleva LI AD - Department of Genetics, Biotechnology, Plant Breeding and Seed Science, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Timiryazevskay str.49, 127550, Moscow, Russia. ludmila.khrustaleva19@gmail.com. AD - Center of Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Listvennichnaya Alleya 5, 127550, Moscow, Russia. ludmila.khrustaleva19@gmail.com. LA - eng PT - Journal Article DEP - 20150702 PL - England TA - BMC Genet JT - BMC genetics JID - 100966978 SB - IM MH - *Chromosomes, Plant MH - In Situ Hybridization, Fluorescence/methods MH - *Pachytene Stage MH - *Physical Chromosome Mapping MH - Rosa/*genetics PMC - PMC4488978 EDAT- 2015/07/03 06:00 MHDA- 2016/03/25 06:00 PMCR- 2015/07/02 CRDT- 2015/07/03 06:00 PHST- 2015/02/11 00:00 [received] PHST- 2015/06/16 00:00 [accepted] PHST- 2015/07/03 06:00 [entrez] PHST- 2015/07/03 06:00 [pubmed] PHST- 2016/03/25 06:00 [medline] PHST- 2015/07/02 00:00 [pmc-release] AID - 10.1186/s12863-015-0233-9 [pii] AID - 233 [pii] AID - 10.1186/s12863-015-0233-9 [doi] PST - epublish SO - BMC Genet. 2015 Jul 2;16:74. doi: 10.1186/s12863-015-0233-9.