PMID- 26136535
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20200930
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 309
IP  - 5
DP  - 2015 Sep
TI  - Dopamine, vesicular transporters, and dopamine receptor expression in rat major 
      salivary glands.
PG  - R585-93
LID - 10.1152/ajpregu.00455.2014 [doi]
AB  - The localization of dopamine stores and the expression and localization of 
      dopamine (DAT) and vesicular monoamine transporters (VMAT) type-1 and -2 and of 
      dopamine D1-like and D2-like receptor subtypes were investigated in rat 
      submandibular, sublingual, and parotid salivary glands by HPLC with 
      electrochemical detection, as well as immunochemical and immunohistochemical 
      techniques. Male Wistar rats of 2 mo of age were used. The highest dopamine 
      levels were measured in the parotid gland, followed by the submandibular and 
      sublingual glands. Western blot analysis revealed DAT, VMAT-1, VMAT-2, and 
      dopamine receptors immunoreactivity in membrane preparations obtained from the 
      three glands investigated. Immunostaining for dopamine and transporters was 
      developed within striated ducts. Salivary glands processed for dopamine receptors 
      immunohistochemistry developed an immunoreaction primarily in striated and 
      excretory ducts. In the submandibular gland, acinar cells displayed strong 
      immunoreactivity for the D2 receptor, while cells of the convoluted granular 
      tubules were negative for both D1-like and D2-like receptors. Parotid glands 
      acinar cells displayed the highest immunoreactivity for both D1 and D2 receptors 
      compared with other salivary glands. The above localization of dopamine and 
      dopaminergic markers investigated did not correspond closely with neuron-specific 
      enolase (NSE) localization. This indicates that at least in part, catecholamine 
      stores and dopaminergic markers are independent from glandular innervation. These 
      findings suggest that rat major salivary glands express a dopaminergic system 
      probably involved in salivary secretion. The stronger immunoreactivity for 
      dopamine transporters and receptors in striated duct cells suggests that the 
      dopaminergic system could regulate not only quality, but also volume and ionic 
      concentration of saliva.
CI  - Copyright (c) 2015 the American Physiological Society.
FAU - Tomassoni, Daniele
AU  - Tomassoni D
AD  - Scuola di Bioscienze e Medicina Veterinaria, Universita di Camerino, Camerino 
      Italy;
FAU - Traini, Enea
AU  - Traini E
AD  - Scuola di Scienze del Farmaco e dei Prodotti della Salute, Universita di 
      Camerino, Camerino Italy; and Dipartimento di Scienze Biomediche e 
      Biotecnologiche, Sezione Biochimica Medica, Universita di Catania, Catania Italy.
FAU - Mancini, Manuele
AU  - Mancini M
AD  - Scuola di Scienze del Farmaco e dei Prodotti della Salute, Universita di 
      Camerino, Camerino Italy; and.
FAU - Bramanti, Vincenzo
AU  - Bramanti V
AD  - Dipartimento di Scienze Biomediche e Biotecnologiche, Sezione Biochimica Medica, 
      Universita di Catania, Catania Italy.
FAU - Mahdi, Syed Sarosh
AU  - Mahdi SS
AD  - Scuola di Scienze del Farmaco e dei Prodotti della Salute, Universita di 
      Camerino, Camerino Italy; and.
FAU - Amenta, Francesco
AU  - Amenta F
AD  - Scuola di Scienze del Farmaco e dei Prodotti della Salute, Universita di 
      Camerino, Camerino Italy; and francesco.amenta@unicam.it.
LA  - eng
PT  - Journal Article
DEP - 20150701
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Receptors, Dopamine)
RN  - 0 (Receptors, Dopamine D1)
RN  - 0 (Receptors, Dopamine D2)
RN  - 0 (Slc18a1 protein, rat)
RN  - 0 (Slc18a2 protein, rat)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Chromatography, High Pressure Liquid
MH  - Dopamine/*analysis
MH  - Dopamine Plasma Membrane Transport Proteins/*analysis
MH  - Dopaminergic Neurons/chemistry
MH  - Immunohistochemistry
MH  - Male
MH  - Parotid Gland/chemistry
MH  - Phosphopyruvate Hydratase/analysis
MH  - Rats, Wistar
MH  - Receptors, Dopamine/*analysis
MH  - Receptors, Dopamine D1/analysis
MH  - Receptors, Dopamine D2/analysis
MH  - Salivary Glands/*chemistry/innervation
MH  - Sublingual Gland/chemistry
MH  - Submandibular Gland/chemistry
MH  - Vesicular Monoamine Transport Proteins/*analysis
OTO - NOTNLM
OT  - dopamine receptors
OT  - immunohistochemistry
OT  - neuron-specific enolase
OT  - salivary glands
OT  - vesicular monoamine transporters
EDAT- 2015/07/03 06:00
MHDA- 2016/04/27 06:00
CRDT- 2015/07/03 06:00
PHST- 2014/10/31 00:00 [received]
PHST- 2015/06/25 00:00 [accepted]
PHST- 2015/07/03 06:00 [entrez]
PHST- 2015/07/03 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
AID - ajpregu.00455.2014 [pii]
AID - 10.1152/ajpregu.00455.2014 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2015 Sep;309(5):R585-93. doi: 
      10.1152/ajpregu.00455.2014. Epub 2015 Jul 1.