PMID- 26136586
OWN - NLM
STAT- MEDLINE
DCOM- 20151027
LR  - 20230216
IS  - 1541-6100 (Electronic)
IS  - 0022-3166 (Linking)
VI  - 145
IP  - 8
DP  - 2015 Aug
TI  - Milk Polar Lipids Affect In Vitro Digestive Lipolysis and Postprandial Lipid 
      Metabolism in Mice.
PG  - 1770-7
LID - 10.3945/jn.115.212068 [doi]
AB  - BACKGROUND: Polar lipid (PL) emulsifiers such as milk PLs (MPLs) may affect 
      digestion and subsequent lipid metabolism, but focused studies on postprandial 
      lipemia are lacking. OBJECTIVE: We evaluated the impact of MPLs on postprandial 
      lipemia in mice and on lipid digestion in vitro. METHODS: Female Swiss mice were 
      gavaged with 150 muL of an oil-in-water emulsion stabilized with 5.7 mg of either 
      MPLs or soybean PLs (SPLs) and killed after 1, 2, or 4 h. Plasma lipids were 
      quantified and in the small intestine, gene expression was analyzed by reverse 
      transcriptase-quantitative polymerase chain reaction. Emulsions were lipolyzed in 
      vitro using a static human digestion model; triglyceride (TG) disappearance was 
      followed by thin-layer chromatography. RESULTS: In mice, after 1 h, plasma TGs 
      tended to be higher in the MPL group than in the SPL group (141 mug/mL vs. 90 
      mug/mL; P = 0.07) and nonesterified fatty acids (NEFAs) were significantly higher 
      (64 mug/mL vs. 44 mug/mL; P < 0.05). The opposite was observed after 4 h with lower 
      TGs (21 mug/mL vs. 35 mug/mL; P < 0.01) and NEFAs (20 mug/mL vs. 32 mug/mL; P < 0.01) 
      in the MPL group compared with the SPL group. This was associated at 4 h with a 
      lower gene expression of apolipoprotein B (Apob) and Secretion Associated, Ras 
      related GTPase 1 gene homolog B (Sar1b), in the duodenum of MPL mice compared 
      with SPL mice (P < 0.05). In vitro, during the intestinal phase, TGs were 
      hydrolyzed more in the MPL emulsion than in the SPL emulsion (decremental AUCs 
      were 1750%/min vs. 180%/min; P < 0.01). MPLs enhance lipid intestinal hydrolysis 
      and promote more rapid intestinal lipid absorption and sharper kinetics of 
      lipemia. CONCLUSIONS: Postprandial lipemia in mice can be modulated by 
      emulsifying with MPLs compared with SPLs, partly through differences in 
      chylomicron assembly, and TG hydrolysis rate as observed in vitro. MPLs may 
      thereby contribute to the long-term regulation of lipid metabolism.
CI  - (c) 2015 American Society for Nutrition.
FAU - Lecomte, Manon
AU  - Lecomte M
AD  - UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, 
      U1060 National Institute of Health and Medical Research (INSERM), National 
      Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary 
      Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition 
      Laboratory, Villeurbanne, France;
FAU - Bourlieu, Claire
AU  - Bourlieu C
AD  - UMR 1253 National Institute for Agricultural Research (INRA), Science & 
      Technology of Milk and Egg, Rennes, France; Agrocampus Ouest, Science & 
      Technology of Milk and Egg, Rennes, France;
FAU - Meugnier, Emmanuelle
AU  - Meugnier E
AD  - U1060 National Institute of Health and Medical Research (INSERM), UMR 1397 
      National Institute for Agricultural Research (INRA), Cardiovascular, Metabolism, 
      Diabetologia and Nutrition Laboratory, Oullins, France; and.
FAU - Penhoat, Armelle
AU  - Penhoat A
AD  - UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, 
      U1060 National Institute of Health and Medical Research (INSERM), National 
      Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary 
      Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition 
      Laboratory, Villeurbanne, France;
FAU - Cheillan, David
AU  - Cheillan D
AD  - UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, 
      U1060 National Institute of Health and Medical Research (INSERM), National 
      Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary 
      Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition 
      Laboratory, Villeurbanne, France; Hereditary Metabolic Diseases Department, East 
      Medical Group, Lyon Civil Hospitals, Lyon, France.
FAU - Pineau, Gaelle
AU  - Pineau G
AD  - UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, 
      U1060 National Institute of Health and Medical Research (INSERM), National 
      Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary 
      Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition 
      Laboratory, Villeurbanne, France;
FAU - Loizon, Emmanuelle
AU  - Loizon E
AD  - U1060 National Institute of Health and Medical Research (INSERM), UMR 1397 
      National Institute for Agricultural Research (INRA), Cardiovascular, Metabolism, 
      Diabetologia and Nutrition Laboratory, Oullins, France; and.
FAU - Trauchessec, Michele
AU  - Trauchessec M
AD  - Hereditary Metabolic Diseases Department, East Medical Group, Lyon Civil 
      Hospitals, Lyon, France.
FAU - Claude, Mathilde
AU  - Claude M
AD  - UMR 1253 National Institute for Agricultural Research (INRA), Science & 
      Technology of Milk and Egg, Rennes, France; Agrocampus Ouest, Science & 
      Technology of Milk and Egg, Rennes, France;
FAU - Menard, Olivia
AU  - Menard O
AD  - UMR 1253 National Institute for Agricultural Research (INRA), Science & 
      Technology of Milk and Egg, Rennes, France; Agrocampus Ouest, Science & 
      Technology of Milk and Egg, Rennes, France;
FAU - Geloen, Alain
AU  - Geloen A
AD  - UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, 
      U1060 National Institute of Health and Medical Research (INSERM), National 
      Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary 
      Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition 
      Laboratory, Villeurbanne, France;
FAU - Laugerette, Fabienne
AU  - Laugerette F
AD  - UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, 
      U1060 National Institute of Health and Medical Research (INSERM), National 
      Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary 
      Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition 
      Laboratory, Villeurbanne, France;
FAU - Michalski, Marie-Caroline
AU  - Michalski MC
AD  - UMR 1397 National Institute for Agricultural Research (INRA), Lyon 1 University, 
      U1060 National Institute of Health and Medical Research (INSERM), National 
      Institute of Applied Science of Lyon, INSA-Lyon, Institute for Multidisciplinary 
      Biochemistry of Lipids, Cardiovascular, Metabolism, Diabetologia and Nutrition 
      Laboratory, Villeurbanne, France; marie-caroline.michalski@insa-lyon.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150701
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Emulsifying Agents)
RN  - 0 (Lecithins)
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Emulsifying Agents
MH  - Female
MH  - Gene Expression Regulation
MH  - Intestine, Small/metabolism
MH  - Lecithins
MH  - Lipid Metabolism/*drug effects
MH  - Lipids/chemistry/*pharmacology
MH  - Lipolysis/*drug effects
MH  - Mice
MH  - Milk/*chemistry
MH  - Postprandial Period
OTO - NOTNLM
OT  - digestion
OT  - emulsion
OT  - food
OT  - lipemia
OT  - nutrition
OT  - sphingomyelin
EDAT- 2015/07/03 06:00
MHDA- 2015/10/28 06:00
CRDT- 2015/07/03 06:00
PHST- 2015/02/10 00:00 [received]
PHST- 2015/06/03 00:00 [accepted]
PHST- 2015/07/03 06:00 [entrez]
PHST- 2015/07/03 06:00 [pubmed]
PHST- 2015/10/28 06:00 [medline]
AID - S0022-3166(22)08821-6 [pii]
AID - 10.3945/jn.115.212068 [doi]
PST - ppublish
SO  - J Nutr. 2015 Aug;145(8):1770-7. doi: 10.3945/jn.115.212068. Epub 2015 Jul 1.