PMID- 26136865
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 9
IP  - 5
DP  - 2015 May
TI  - Increased glyceraldehyde-3-phosphate dehydrogenase expression indicates higher 
      survival rates in male patients with hepatitis B virus-accociated hepatocellular 
      carcinoma and cirrhosis.
PG  - 1597-1604
AB  - Elevated expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been 
      reported in different human malignancies. To understand its role in hepatitis B 
      virus (HBV) infection-associated hepatocellular carcinoma (HCC), the expression 
      of GAPDH was quantitatively measured in a cohort of 72 male HCC patients without 
      preoperative treatment, all with evidence of chronic HBV infection. Using 
      C-terminal banding protein 1 (CTBP1) or hypoxanthine phosphori-bosyltransferase 1 
      (HPRT1) as reference genes, the level of GAPDH mRNA in tumor tissue was found to 
      be significantly higher compared with that in paired non tumor tissues (P=0.0087 
      for CTBP1; P=0.0116 for HPRT1). Accordingly, compared with the non-tumor tissue, 
      37.5% (27/72) of patients' tumor tissues had a more than 2-fold increase of GAPDH 
      expression. Furthermore, following knockdown GAPDH expression via siRNA transient 
      transfection, HepG2 cells exhibited enhanced resistance to cytosine arabinoside 
      (IC(50), 308.28 microM vs. 67.68 microM in the control; P=0.01). Notably, higher GAPDH 
      expression was significantly associated with lower liver fibrosis score 
      (P=0.0394) and a tendency towards higher survival rates for patients with HCC. To 
      the best of our knowledge, the present study is the first study to report that 
      the elevated expression levels of GAPDH in HCC tumor tissue may be relevant to an 
      improved fibrosis score and survival probability in male patients with HBV 
      infection; however, the underlying mechanism requires further investigation.
FAU - Liu, Shuang
AU  - Liu S
AD  - Beijing Artificial Liver Treatment and Training Center, Beijing Youan Hospital, 
      Capital Medical University, Beijing 100069, P.R. China.
FAU - Zhu, Pengfei
AU  - Zhu P
AD  - Beijing Artificial Liver Treatment and Training Center, Beijing Youan Hospital, 
      Capital Medical University, Beijing 100069, P.R. China.
FAU - Zhang, Ling
AU  - Zhang L
AD  - Department of Hepatobiliary Surgery, Henan Cancer Hospital, Zhengzhou, Henan 
      450008, P.R. China.
FAU - Li, Zhuo
AU  - Li Z
AD  - Beijing Artificial Liver Treatment and Training Center, Beijing Youan Hospital, 
      Capital Medical University, Beijing 100069, P.R. China.
FAU - Lv, Quanjun
AU  - Lv Q
AD  - Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou 
      University, Zhengzhou, Henan 450052, P.R. China.
FAU - Zheng, Sujun
AU  - Zheng S
AD  - Beijing Artificial Liver Treatment and Training Center, Beijing Youan Hospital, 
      Capital Medical University, Beijing 100069, P.R. China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Beijing Artificial Liver Treatment and Training Center, Beijing Youan Hospital, 
      Capital Medical University, Beijing 100069, P.R. China.
FAU - Lu, Fengmin
AU  - Lu F
AD  - Department of Microbiology and Infectious Disease Center, Peking University 
      Health Science Center, Beijing 100086, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20150224
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4471696
OTO - NOTNLM
OT  - chemotherapy
OT  - glyceraldehyde-3-phosphate dehydrogenase
OT  - hepatitis B virus
OT  - hepatocellular carcinoma
OT  - liver cirrhosis
EDAT- 2015/07/03 06:00
MHDA- 2015/07/03 06:01
PMCR- 2015/02/24
CRDT- 2015/07/03 06:00
PHST- 2014/05/04 00:00 [received]
PHST- 2015/01/15 00:00 [accepted]
PHST- 2015/07/03 06:00 [entrez]
PHST- 2015/07/03 06:00 [pubmed]
PHST- 2015/07/03 06:01 [medline]
PHST- 2015/02/24 00:00 [pmc-release]
AID - ETM-0-0-2309 [pii]
AID - 10.3892/etm.2015.2309 [doi]
PST - ppublish
SO  - Exp Ther Med. 2015 May;9(5):1597-1604. doi: 10.3892/etm.2015.2309. Epub 2015 Feb 
      24.