PMID- 26138242 OWN - NLM STAT- MEDLINE DCOM- 20160829 LR - 20200401 IS - 1543-706X (Electronic) IS - 1071-2690 (Linking) VI - 51 IP - 10 DP - 2015 Nov TI - Nucleostemin exerts anti-apoptotic function via p53 signaling pathway in cardiomyocytes. PG - 1064-71 LID - 10.1007/s11626-015-9934-7 [doi] AB - Cardiomyocytes apoptosis following reperfusion injury causes irreversible damage to cardiac function. Understanding the mechanisms underlying cardiomyocytes death under these conditions can be helpful to identify strategies to abrogate such detrimental effects. Stem cell-specific proteins and regulatory pathways become important in understanding reparative processes in the myocardium. One such regulatory protein named nucleostemin (NS) has vital roles in cardiac ischemia. Although the relationship between NS and cell apoptosis has been studied, it is unknown how NS is controlled and how it participates in cardiomyocytes apoptosis induced by ischemia reperfusion (I/R). In the present study, we aimed to investigate the direct role of NS in myocardial I/R. In vivo, NS was highly expressed in cardiac tissues after I/R. Double immunofluorescent staining showed that NS located in the nucleolar of cardiomyocytes and correlated with cardiomyocytes apoptosis. Furthermore, in vitro primary rat cardiomyocytes increased NS expression induced by hypoxia-reoxygenation (H/R) treatment, in line with results in vivo. Suppression of NS expression by siNS promoted the expression of terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL)-positive cells p53 and cleaved caspase-3, which demonstrates I/R may require increased expression of NS to suppress p53 activation and maintain cardiomyocytes survival. FAU - Zhang, Chi AU - Zhang C AD - Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. FAU - Shi, Jiahai AU - Shi J AD - Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. FAU - Qian, Long AU - Qian L AD - Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. FAU - Zhang, Chao AU - Zhang C AD - Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. FAU - Wu, Kunpeng AU - Wu K AD - Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. FAU - Yang, Chen AU - Yang C AD - Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. FAU - Yan, Daliang AU - Yan D AD - Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. FAU - Wu, Xiang AU - Wu X AD - Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. ntwx0513@163.com. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. ntwx0513@163.com. FAU - Liu, Xiaojuan AU - Liu X AD - Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. ntlxj0305@163.com. AD - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. ntlxj0305@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150703 PL - Germany TA - In Vitro Cell Dev Biol Anim JT - In vitro cellular & developmental biology. Animal JID - 9418515 RN - 0 (Carrier Proteins) RN - 0 (Gnl3 protein, rat) RN - 0 (Nuclear Proteins) RN - 0 (RNA, Small Interfering) RN - 0 (Tumor Suppressor Protein p53) RN - EC 3.4.22.- (Caspase 3) RN - EC 3.6.1.- (GTP-Binding Proteins) SB - IM MH - Animals MH - Apoptosis/genetics/*physiology MH - Cardiac Surgical Procedures MH - Carrier Proteins/genetics/*metabolism MH - Caspase 3/biosynthesis MH - Cells, Cultured MH - GTP-Binding Proteins MH - In Situ Nick-End Labeling MH - Myocardial Reperfusion Injury/*pathology MH - Myocardium/*pathology MH - Myocytes, Cardiac/metabolism MH - Nuclear Proteins/genetics/*metabolism MH - RNA Interference MH - RNA, Small Interfering/genetics MH - Random Allocation MH - Rats MH - Rats, Sprague-Dawley MH - Signal Transduction/physiology MH - Tumor Suppressor Protein p53/biosynthesis/*metabolism OTO - NOTNLM OT - Apoptosis OT - Myocardial ischemia reperfusion OT - Nucleostemin OT - p53 EDAT- 2015/07/04 06:00 MHDA- 2016/08/30 06:00 CRDT- 2015/07/04 06:00 PHST- 2015/04/21 00:00 [received] PHST- 2015/06/14 00:00 [accepted] PHST- 2015/07/04 06:00 [entrez] PHST- 2015/07/04 06:00 [pubmed] PHST- 2016/08/30 06:00 [medline] AID - 10.1007/s11626-015-9934-7 [pii] AID - 10.1007/s11626-015-9934-7 [doi] PST - ppublish SO - In Vitro Cell Dev Biol Anim. 2015 Nov;51(10):1064-71. doi: 10.1007/s11626-015-9934-7. Epub 2015 Jul 3.