PMID- 26138484
OWN - NLM
STAT- MEDLINE
DCOM- 20151124
LR  - 20211203
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 43
IP  - 14
DP  - 2015 Aug 18
TI  - Phosphorylation regulates the Star-PAP-PIPKIalpha interaction and directs specificity 
      toward mRNA targets.
PG  - 7005-20
LID - 10.1093/nar/gkv676 [doi]
AB  - Star-PAP is a nuclear non-canonical poly(A) polymerase (PAP) that shows 
      specificity toward mRNA targets. Star-PAP activity is stimulated by lipid 
      messenger phosphatidyl inositol 4,5 bisphoshate (PI4,5P2) and is regulated by the 
      associated Type I phosphatidylinositol-4-phosphate 5-kinase that synthesizes 
      PI4,5P2 as well as protein kinases. These associated kinases act as coactivators 
      of Star-PAP that regulates its activity and specificity toward mRNAs, yet the 
      mechanism of control of these interactions are not defined. We identified a 
      phosphorylated residue (serine 6, S6) on Star-PAP in the zinc finger region, the 
      domain required for PIPKIalpha interaction. We show that S6 is phosphorylated by CKIalpha 
      within the nucleus which is required for Star-PAP nuclear retention and 
      interaction with PIPKIalpha. Unlike the CKIalpha mediated phosphorylation at the 
      catalytic domain, Star-PAP S6 phosphorylation is insensitive to oxidative stress 
      suggesting a signal mediated regulation of CKIalpha activity. S6 phosphorylation 
      together with coactivator PIPKIalpha controlled select subset of Star-PAP target 
      messages by regulating Star-PAP-mRNA association. Our results establish a novel 
      role for phosphorylation in determining Star-PAP target mRNA specificity and 
      regulation of 3'-end processing.
CI  - (c) The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic 
      Acids Research.
FAU - Mohan, Nimmy
AU  - Mohan N
AD  - Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, 
      Poojappura, Trivandrum 695014, India.
FAU - Sudheesh, A P
AU  - Sudheesh AP
AD  - Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, 
      Poojappura, Trivandrum 695014, India.
FAU - Francis, Nimmy
AU  - Francis N
AD  - Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, 
      Poojappura, Trivandrum 695014, India.
FAU - Anderson, Richard
AU  - Anderson R
AD  - School of Medicine and Public Health, University of Wisconsin-Madison, WI 53706, 
      USA.
FAU - Laishram, Rakesh S
AU  - Laishram RS
AD  - Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, 
      Poojappura, Trivandrum 695014, India laishram@rgcb.res.in.
LA  - eng
GR  - IA/I/12/1/500508/WTDBT_/DBT-Wellcome Trust India Alliance/India
GR  - R01 GM051968/GM/NIGMS NIH HHS/United States
GR  - R35 GM134955/GM/NIGMS NIH HHS/United States
GR  - IA/I/1/505008/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150702
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Phosphatidylinositol 4,5-Diphosphate)
RN  - 0 (RNA, Messenger)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.68 (1-phosphatidylinositol-4-phosphate 5-kinase)
RN  - EC 2.7.11.1 (Casein Kinase I)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
RN  - EC 2.7.7.- (TUT1 protein, human)
RN  - EC 2.7.7.19 (Polynucleotide Adenylyltransferase)
SB  - IM
MH  - Casein Kinase I/metabolism
MH  - Cell Nucleus/enzymology
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Nucleotidyltransferases
MH  - Oxidative Stress
MH  - Phosphatidylinositol 4,5-Diphosphate/metabolism
MH  - Phosphorylation
MH  - Phosphotransferases (Alcohol Group Acceptor)/*metabolism
MH  - Polynucleotide Adenylyltransferase/chemistry/*metabolism
MH  - Protein Binding
MH  - *RNA 3' End Processing
MH  - RNA, Messenger/*metabolism
MH  - Serine/metabolism
MH  - Signal Transduction
MH  - Zinc Fingers
PMC - PMC4538844
EDAT- 2015/07/04 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/07/02
CRDT- 2015/07/04 06:00
PHST- 2015/06/19 00:00 [accepted]
PHST- 2015/01/28 00:00 [received]
PHST- 2015/07/04 06:00 [entrez]
PHST- 2015/07/04 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/07/02 00:00 [pmc-release]
AID - gkv676 [pii]
AID - 10.1093/nar/gkv676 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2015 Aug 18;43(14):7005-20. doi: 10.1093/nar/gkv676. Epub 2015 
      Jul 2.